ACVR1 Rabbit Monoclonal Antibody for WB and ELISA - Q04771

Core Product Specifications and Parameters

Biological Background: Activin Receptor Type-1 (ACVR1) Function and Localization

• ACVR1, also known as ALK2, ACTR-I, SKR1, or TSR-I, is a bone morphogenetic protein (BMP) type I receptor encoded by the ACVR1 gene (ACVRLK2).

• It mediates BMP signaling essential for bone, heart, cartilage, nervous, and reproductive system development and regulation. Related references: PMID:20628059, PMID:22977237

• Forms heterotetrameric complexes with type II receptors AMHR2, ACVR2A, or ACVR2B to respond to ligands. Related references: PMID:17911401

• Ligand binding (e.g., BMP7, GDF2/BMP9) induces type II receptor-mediated transphosphorylation of the ACVR1 intracellular domain, activating its kinase function. Related references: PMID:25354296

• Activated ACVR1 phosphorylates SMAD1/5/8 proteins, triggering canonical BMP signal transduction. Related references: PMID:9748228

• Suppresses TGFβ/activin pathway by interfering with activin binding to its type II receptor. Related references: PMID:17911401

• Can activate non-canonical p38 MAPK pathways.

• Localizes to the plasma membrane; expressed in parenchymal cells, endothelial cells, fibroblasts, and tumor-derived epithelial cells.

• Classified as a glycoprotein and phosphoprotein with ATP-binding and metal-binding kinase properties.

Experimental Guidance and Technical Tips

• The immunogen (aa 410–509) lies within the intracellular kinase domain of human ACVR1; this design supports specific detection of full-length ACVR1 protein.
• For Western blot, a band near 57 kDa is expected; use ACVR1-expressing cell lines (e.g., HeLa, HEK293) as positive controls.
• Cross-reactivity with mouse and rat ACVR1 is reported; verify signal specificity when testing samples from these species.
• For ELISA, optimize coating antigen (peptide or recombinant ACVR1 fragment) and antibody dilution to achieve optimal signal-to-noise ratio.
• Consider using blocking buffers and incubation conditions tailored to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, serving diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This ACVR1 rabbit monoclonal antibody supports BMP pathway studies, and we supply validated antibody pairs, bulk monoclonals and polyclonals, and ancillary reagents for scalable assay development. For hard-to-find targets, our expert team quickly sources rare raw materials to keep your projects on track.