Anti-α-Actin-1 (ACTA1) Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P68133

Core Product Specifications and Parameters

Biological Background: α-Actin-1 (ACTA1) Function and Localization

• α-Actin-1 (ACTA1) is a skeletal muscle-specific actin isoform, belonging to the highly conserved actin family that is central to cell motility and cytoskeletal architecture.
• It serves as a core component of the thin filament in sarcomeres, essential for muscle contraction and force generation.
• ACTA1 is one of six actin isoforms; the others include cardiac muscle α-actin, smooth muscle α- and γ-actins, and cytoplasmic β- and γ-actins.
• Subcellularly, ACTA1 localizes predominantly to the cytoplasmic cytoskeleton, particularly in skeletal muscle fibers.
• The protein undergoes multiple post-translational modifications such as acetylation, methylation, oxidation, and isopeptide bond formation, which may regulate its polymerization and interactions.
• Mutations in ACTA1 are associated with nemaline myopathy and other congenital myopathies, highlighting its critical role in muscle structural integrity.
• It possesses ATP-binding and nucleotide-binding sites, indicative of its enzymatic hydrolase activity during actin dynamics.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1-100) of human ACTA1, a sequence well-conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, a band of approximately 42 kDa is expected; use skeletal muscle tissue lysates as a positive control.
• In immunofluorescence (IF/ICC, IF-P), ensure cells or tissue sections are adequately permeabilized to allow antibody access to cytoplasmic actin filaments.
• For ELISA applications, coat plates with the synthetic peptide or recombinant protein and titrate the antibody to determine optimal working dilution.
• It is advisable to validate the antibody's performance in your specific experimental system, as reactivity may vary with sample preparation and fixation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials for diagnostic developers and research laboratories, providing one-stop access to antibodies, antigens, and ancillary reagents. With a focus on muscle biology and neuromuscular disorders, this anti-ACTA1 rabbit monoclonal antibody exemplifies our commitment to supporting rare target sourcing and assay development. From concept to clinic, our integrated supply chain and technical expertise help accelerate your progress.