ACE1 Rabbit Monoclonal Antibody for WB, IP, ELISA - P12821

Core Product Specifications and Parameters

Biological Background: ACE1 Function and Localization

• ACE1 is a dipeptidyl carboxypeptidase that removes C-terminal dipeptides from various circulating hormones, playing key roles in blood pressure regulation, electrolyte homeostasis, and synaptic plasticity. Related references: PMID:15615692, PMID:20826823, PMID:2558109
• The enzyme contains two homologous catalytic domains with distinct substrate specificities; the N-domain preferentially degrades N-acetyl-SDKP and amyloid-beta, while the C-domain processes angiotensin I and cholecystokinin. Related references: PMID:10913258, PMID:1320019, PMID:1851160, PMID:10336644, PMID:19773553, PMID:7876104
• In the renin-angiotensin system, ACE1 converts angiotensin I to the potent vasoconstrictor angiotensin II and simultaneously inactivates the vasodilator bradykinin, thereby increasing blood pressure. Related references: PMID:11432860, PMID:1851160, PMID:19773553, PMID:15615692, PMID:2558109, PMID:4322742
• ACE1 regulates synaptic transmission by cleaving neuropeptides such as substance P, neurotensin, and enkephalins, and modulates cannabinoid signaling through degradation of hemopressin. Related references: PMID:15615692, PMID:6208535, PMID:6270633, PMID:2982830, PMID:656131, PMID:18077343
• It degrades the hemoregulatory peptide N-acetyl-SDKP, influencing hematopoietic stem cell differentiation, and participates in amyloid-beta clearance, thereby reducing plaque formation. Related references: PMID:26403559, PMID:7876104, PMID:8257427, PMID:11604391, PMID:16154999, PMID:19773553
• A soluble form is released into plasma after proteolytic shedding, and a testis-specific isoform produced from an alternative promoter is essential for male fertility. Related references: PMID:1651327, PMID:1668266, PMID:24297181
• ACE1 is a transmembrane zinc metalloprotease with post-translational modifications including phosphorylation and glycosylation; it localizes to the cell membrane and cytoplasm, and is also secreted.
• Ubiquitously expressed with highest levels in lung, kidney, heart, gastrointestinal system, and prostate; the testis-specific isoform is found in spermatocytes and adult testis.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1200–1306) is located in the C-terminal domain, which is conserved in mouse and rat, consistent with the reported cross-reactivity.
• For western blotting, the full-length somatic ACE1 is predicted at ~150 kDa; consider using reducing conditions and validate in the relevant sample system.
• When performing immunoprecipitation or ELISA, perform initial pilot experiments to determine optimal antibody dilution and confirm specificity in your particular matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined path to IVD raw materials for cardiovascular and neurobiology research, including validated antibody pairs, monoclonal/polyclonal antibodies such as this ACE1 antibody, and ancillary reagents. Our supply capabilities cover bulk orders and rare target sourcing, complemented by technical support to help you move from concept to clinic efficiently.