AKT1 Rabbit Monoclonal Antibody for WB, IHC-P, IP - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1, also known as RAC-alpha serine/threonine-protein kinase, Protein kinase B (PKB), or Proto-oncogene c-Akt, is a member of the AGC kinase family.
• AKT1 is one of three closely related AKT kinases (AKT1, AKT2, AKT3) that regulate key cellular processes including metabolism, proliferation, cell survival, growth, and angiogenesis.
  Related references: PMID:11882383 PMID:15526160 PMID:15861136
• It serves as a central downstream effector of phosphatidylinositol 3-kinase (PI3K) signaling, mediating the effects of growth factors such as insulin, IGF1, EGF, and PDGF.
  Related references: PMID:12176338 PMID:12964941
• AKT1 phosphorylates a broad spectrum of substrates (over 100 reported) to influence diverse pathways: for example, it inactivates GSK3 to promote glycogen synthesis, phosphorylates FOXO transcription factors leading to their cytoplasmic retention and inhibition of pro-apoptotic gene expression, activates mTORC1 via TSC2 phosphorylation, and phosphorylates BAD to suppress apoptosis.
  Related references: PMID:11882383 PMID:15526160 PMID:21432781
• Subcellular localization: AKT1 is found in the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, reflecting its involvement in multiple signaling compartments.
• Tissue expression: ubiquitously expressed in all human cell types analyzed; increased protein levels observed in prostate and breast cancers during progression.
• Post-translational modifications: AKT1 undergoes phosphorylation (activation), acetylation, ubiquitination, and disulfide bond formation, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning residues 381–480 of human AKT1 (C-terminal region). For Western blotting, expect a specific band around 56 kDa; however, note that AKT1 has multiple phosphorylation and other modifications that may alter its electrophoretic mobility.
• For immunohistochemistry (IHC-P), antigen retrieval optimization is recommended; test on known positive tissues (e.g., human prostate or breast cancer sections) and include appropriate controls.
• In immunoprecipitation (IP) and ELISA, the rabbit monoclonal format may provide high specificity and batch-to-batch consistency; validate the antibody in the relevant sample matrix.
• Cross-reactivity with mouse and rat AKT1 is indicated, but endogenous expression levels vary by tissue; consider verifying reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is dedicated to supplying diagnostic manufacturers and research laboratories with a comprehensive portfolio of high-quality IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For AKT1-targeted research and assay development in cancer, metabolism, and cellular signaling, we offer efficient sourcing support—helping you secure the critical reagents needed, including rare or custom targets, to move your projects from concept to clinic.