KO Validated Anti-Alpha-synuclein Monoclonal Antibody for WB, IHC, IF, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein is a neuronal protein highly expressed in presynaptic terminals of the central nervous system, principally in brain.
• It regulates synaptic vesicle trafficking and neurotransmitter release by enhancing vesicle priming, fusion, and dilation of exocytotic fusion pores, and increases local Ca2+ release from microdomains to promote exocytosis. Related references: PMID:28288128 PMID:30404828
• As a molecular chaperone in its multimeric membrane-bound state, it assists in folding synaptic SNARE proteins and maintains SNARE-complex assembly during aging. Related references: PMID:20798282
• It modulates dopamine neurotransmission by associating with the dopamine transporter (DAT1). Related references: PMID:26442590
• Subcellular localization includes cytoplasm, membrane, nucleus, synapse, and it is also secreted; found in cell projections such as axons.
• It is implicated in neurodegenerative diseases such as Parkinson's and Alzheimer's, with keywords including amyloid, neurodegeneration, and Parkinson disease.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human alpha-synuclein (aa 1-140), suggesting broad epitope coverage. Consider that it may detect multiple isoforms if the epitope is conserved.
• For Western blot (WB), an expected band at approximately 14 kDa is predicted; validate with appropriate positive controls such as brain tissue lysates from human, mouse, or rat.
• In immunohistochemistry (IHC) and immunofluorescence (IF), antigen retrieval may be necessary; test on brain sections where alpha-synuclein is highly expressed.
• The knockout (KO) validation indicates enhanced specificity; include proper controls in your experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering comprehensive support from early-stage research to clinical application. For alpha-synuclein and Parkinson's disease research, we supply validated monoclonal antibodies, matched antibody pairs, bulk reagents, and custom sourcing to accelerate your diagnostic development.