Anti-Acetyl-CoA Carboxylase Rabbit Polyclonal Antibody for WB, ELISA - Q13085 / O00763

Core Product Specifications and Parameters

Biological Background: Acetyl-CoA Carboxylase Function and Localization

• Acetyl-CoA carboxylase 1 (ACC1), encoded by the ACACA gene, is the primary cytosolic enzyme that initiates de novo fatty acid biosynthesis by converting acetyl-CoA to malonyl-CoA.
• The carboxylation reaction is a two-step process: ATP-dependent biotin carboxylation on the biotin carboxyl carrier domain, followed by transfer of the carboxyl group to acetyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443). Related references: PMID:20457939 PMID:20952656 PMID:29899443
• ACC1 belongs to the biotin-dependent carboxylase family and functions as a multifunctional enzyme with allosteric regulation, playing a critical role in lipid metabolism.
• The protein localizes predominantly in the cytoplasm and cytosol, consistent with its role in fatty acid synthesis.
• Tissue expression analysis shows ACC1 in brain, placenta, skeletal muscle, renal, pancreatic, and adipose tissues, with low levels in lung and absence in liver.
• Post-translational modifications include acetylation and phosphorylation, which regulate its activity in response to metabolic signals.

Experimental Guidance and Technical Tips

• For Western blotting, consider the high molecular weight of ACC proteins (265–276 kDa); use appropriate gel percentages and transfer conditions for large proteins.
• As a polyclonal antibody raised against a synthetic peptide, it may detect both ACC1 and ACC2; validate specificity in your sample system using known controls.
• For ELISA, this antibody can be used as a capture or detection reagent; optimal dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This anti-Acetyl-CoA Carboxylase antibody supports research in fatty acid metabolism, obesity, and cancer, and is part of our broad portfolio of metabolic pathway tools. With one-stop sourcing from concept to clinic, we help streamline your supply chain and accelerate development.