Anti-JAK1 Rabbit Monoclonal Antibody for WB and ELISA - P23458

Core Product Specifications and Parameters

Biological Background: JAK1 Function and Localization

• JAK1 is a non-membrane spanning protein tyrosine kinase that phosphorylates signal-transducing subunits of cytokine receptors, including IL2RB, IL10RA, IFNAR2, IL6ST, LIFR, OSMR, and IL31RA. Related references: PMID:11909529 | PMID:12133952 | PMID:15194700
• It plays a key role in the IFN-alpha/beta/gamma signaling pathway, phosphorylating IFNAR2 upon interferon binding and creating docking sites for STAT proteins. Related references: PMID:7759950 | PMID:16239216 | PMID:28111307 | PMID:32750333
• JAK1 directly phosphorylates STAT proteins and transactivates other JAK kinases, amplifying STAT signaling. Related references: PMID:8232552 | PMID:16239216 | PMID:32750333
• It is involved in the MT-RNR2/humanin-mediated signaling pathway leading to STAT3 phosphorylation. Related references: PMID:27384491
• JAK1 mediates oncostatin-M signaling via type I (LIFR-IL6ST) and type II (OSMR-IL6ST) receptor complexes. Related references: PMID:9188471
• It participates in interleukin-31 signaling through the OSMR-IL31RA receptor complex. Related references: PMID:15194700
• JAK1 localizes to the endomembrane system.
• It is expressed at higher levels in primary colon tumors compared to normal colon tissue, while metastatic colon tumors show expression levels comparable to normal tissue.
• Keywords include kinase, SH2 domain, ATP-binding, magnesium, membrane, metal-binding, nucleotide-binding, phosphoprotein, Ubl conjugation, and acetylation; molecular weight 133 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 721–820 of human JAK1; consider verifying reactivity with your specific sample matrix.
• Recommended primary applications include Western blot (predicted band ~133 kDa) and ELISA; optimal dilutions should be determined experimentally.
• Cross-reactivity is reported for human, mouse, and rat; validate performance in the relevant species and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio integrates validated antibodies, antigens, and ancillary reagents to streamline IVD raw material procurement across development phases. With a focus on kinase signaling and cytokine pathways, we provide reliable access to JAK1 monoclonal antibodies and related critical raw materials. Our sourcing expertise supports your assay development from concept to clinic.