Anti-ACC1 Polyclonal Antibody for WB, IHC-P, ELISA - Q13085

Core Product Specifications and Parameters

Biological Background: ACC1 Function and Localization

• Acetyl-CoA carboxylase 1 (ACC1), encoded by the ACACA gene, is the rate-limiting enzyme in de novo fatty acid biosynthesis, catalyzing the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA (PubMed:20457939, PubMed:20952656, PubMed:29899443). Related references: PMID:20457939 PMID:20952656 PMID:29899443
• The enzyme is a large multifunctional protein containing biotin carboxyl carrier, biotin carboxylase, and carboxyltransferase domains, and requires biotin as a cofactor.
• ACC1 localizes predominantly to the cytosol, where it provides the malonyl-CoA substrate for fatty acid synthesis.
• Tissue expression includes brain, placenta, skeletal muscle, renal, pancreatic, and adipose tissues, with low levels in lung and absence in liver.
• Post-translational modifications include acetylation and phosphorylation, which regulate enzymatic activity and polymer formation.
• ACC1 is a metal-dependent ligase, utilizing magnesium or manganese for catalytic activity, and is subject to allosteric regulation by citrate and palmitoyl-CoA.

Experimental Guidance and Technical Tips

• For Western blot (WB), given the large protein size (~266 kDa), use a low percentage SDS-PAGE gel (e.g., 6%) and extended transfer time to ensure efficient blotting.
• For immunohistochemistry (IHC-P), consider antigen retrieval methods such as heat-induced epitope retrieval in citrate buffer (pH 6.0) to expose the immunogen epitope located in the C-terminal region (aa 1190-1340).
• In ELISA, titrate the antibody appropriately and validate against the recombinant immunogen or cell lysates to confirm specificity.
• The antibody is raised against a conserved region; cross-reactivity with rat ACC1 has been demonstrated, but sequence alignment with other species is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering a comprehensive portfolio of validated antibodies, including polyclonal and monoclonal options targeting key metabolic enzymes like ACC1. Whether you need bulk ancillary reagents, OEM-scale antibody production, or assistance sourcing rare-target raw materials for lipid metabolism research, CamelBio streamlines your supply chain from concept to clinic. Leverage our one-stop service to accelerate your diagnostic development with reliable, high-quality reagents backed by technical expertise.