Acetyl-BRD4 (K332) Rabbit Polyclonal Antibody (SWISS O60885) | WB, ELISA - BRD4 Acetylation Detection

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 (Bromodomain-containing protein 4) is a chromatin reader that recognizes acetylated histones, playing a central role in epigenetic memory and transcription regulation. It remains associated with acetylated chromatin throughout the cell cycle and helps preserve chromatin architecture for postmitotic gene transcription. During interphase, BRD4 recruits the P-TEFb complex to promoters and distal enhancers, facilitating RNA polymerase II CTD phosphorylation and transcriptional elongation. It also mediates NF-kappa-B activation by binding acetylated RELA and regulates p53-dependent transcription.
  Related references: PMID:16109376, PMID:16109377, PMID:19103749, PMID:23086925, PMID:23317504, PMID:23589332, PMID:24360279, PMID:29176719

• An isoform of BRD4 functions as a chromatin insulator in the DNA damage response by recruiting condensin-2 to acetylated histones, thereby limiting H2A.X phosphorylation spreading.
  Related references: PMID:23728299

• BRD4 localizes predominantly to the nucleus and associates with acetylated chromatin and mitotic chromosomes. Its chromatin binding is regulated by histone acetylation status, influenced by signals such as JNK pathway activation.
  Related references: PMID:16109376, PMID:21890894, PMID:25593309

• BRD4 is ubiquitously expressed and subject to extensive post-translational modifications, including phosphorylation at multiple serine residues (e.g., S470, S484, S488, S492, S494, S498, S499, S503, S601, S1117, S1126, S1201, S1204) and acetylation at K1111. This antibody specifically detects acetylation at Lys332, a modification not yet fully characterized in UniProt but designed based on the immunogen peptide.
  Related references: PMID:12543779, PMID:18669648, PMID:19608861, PMID:23317504

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide surrounding Lys332 of human BRD4, designed to ensure specific recognition of the acetylated form. For Western blot, consider using nuclear extracts from human or mouse cell lines and validate specificity with peptide competition assays using the acetylated and non-acetylated peptides.
• For ELISA, the acetylated peptide may serve as the coating antigen. Include appropriate controls (non-acetylated peptide, blank wells) and optimize coating concentrations for best signal-to-noise ratio.
• Antibody reactivity may depend on acetylation levels, which can vary with cell treatment (e.g., HDAC inhibitors). Consider stimulating acetylation to enhance detection.
• Validate antibody performance in your specific sample matrix; recommended starting concentration ranges should be determined empirically.
• Avoid repeated freeze-thaw cycles; aliquot and store at -20°C or as recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD raw-material procurement, offering a comprehensive portfolio of antibodies and reagents for diagnostic development and life science research. This acetyl-BRD4-K332 antibody supports studies in epigenetics and transcriptional regulation, and complements our range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. We streamline your supply chain from concept to clinic, ensuring reliable access to high-quality raw materials for your assay needs.