Phospho-BRD4-S498/S499 Rabbit Polyclonal Antibody for WB, ELISA - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 is a chromatin reader that recognizes and binds acetylated histones, playing a key role in epigenetic memory transmission across cell divisions and transcription regulation (PubMed:20871596, PubMed:23086925, PubMed:23317504). Related references: PMID:20871596, PMID:23086925, PMID:23317504
• It remains associated with acetylated chromatin throughout the cell cycle, providing epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining chromatin structure (PubMed:22334664, PubMed:23317504, PubMed:23589332). Related references: PMID:22334664, PMID:23317504, PMID:23589332
• During interphase, BRD4 regulates transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters and enhancers, facilitating RNA polymerase II phosphorylation (PubMed:16109376, PubMed:16109377, PubMed:19596240). Related references: PMID:16109376, PMID:16109377, PMID:19596240
• It also acts as a chromatin insulator in the DNA damage response pathway, inhibiting DNA damage signaling by recruiting the condensin-2 complex to acetylated histones and limiting H2AX phosphorylation spread.
• BRD4 recognizes acetylated RELA, leading to recruitment of P-TEFb and activation of NF-kappa-B (PubMed:19103749). Related references: PMID:19103749
• Following phosphorylation by CK2, BRD4 is recruited to p53/TP53-specific target promoters, regulating p53-mediated transcription (PubMed:23317504). Related references: PMID:23317504
• Subcellular localization is primarily nuclear, associated with chromosomes throughout the cell cycle.
• Ubiquitously expressed across tissues.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide around S498 & S499 of human BRD4; therefore, it is predicted to specifically recognize the phosphorylated form of BRD4.
• For Western blotting, consider using phosphatase inhibitors during lysis and treat samples with alkaline phosphatase to confirm phosphorylation-specific detection.
• For ELISA, the antibody may be used for capturing or detecting phosphorylated BRD4; appropriate peptide blocking could enhance specificity.
• Validate antibody performance in relevant human cell lines or tissues expressing BRD4, such as HeLa or Jurkat cells, as BRD4 is ubiquitously expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For BRD4-related research and epigenetic diagnostic development, we offer one-stop sourcing of phosphorylated and total BRD4 antibodies, along with technical support from concept to clinic. Explore our catalog to accelerate your transcription and DNA damage pathway studies.