Anti-CREB3L2 Rabbit Polyclonal Antibody for WB, ELISA - Q70SY1

Core Product Specifications and Parameters

Biological Background: CREB3L2 Function and Localization

• CREB3L2 (also known as BBF2H7) is a transcription factor involved in the unfolded protein response (UPR) localized to the endoplasmic reticulum (ER) membrane under basal conditions.
• Upon ER stress, CREB3L2 is transported to the Golgi and cleaved by S1P and S2P proteases, releasing its N-terminal domain to translocate to the nucleus and activate transcription of target genes.
• The protein plays a critical role in chondrogenesis by activating SEC23A transcription, promoting secretion of cartilage matrix proteins, and possibly regulating ER biogenesis-related genes.
• In neuroblastoma cells, CREB3L2 protects against ER stress-induced death and activates transcription by directly binding to the CRE site. Related references: PMID:17178827
• CREB3L2 is widely expressed, with highest levels in placenta, lung, spleen, and intestine, and shows nuclear localization following activation.
• UniProt keywords highlight its involvement in chromosomal rearrangement and development, as well as its proto-oncogenic potential; it undergoes phosphorylation, glycosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-247), which includes the DNA-binding and transcription activation domains; the antibody may recognize both full-length and cleaved forms of CREB3L2.
• For Western blot, expect a prominent band near 57 kDa under reducing conditions; under ER stress conditions, additional cleaved fragments may be observed.
• When using ELISA, consider optimizing the antibody concentration and pairing as a capture or detection reagent with appropriate standards.
• As this is a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, offering validated antibodies like this anti-CREB3L2 polyclonal antibody for research into ER stress and UPR pathways. We supply optimized monoclonal/polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to accelerate diagnostic development across diverse targets. With expertise in rare-target procurement, CamelBio bridges the gap between concept and clinic for diagnostic manufacturers and research laboratories.