Anti-BDKRB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P30411

Core Product Specifications and Parameters

Biological Background: B2 Bradykinin Receptor Function and Localization

• B2 bradykinin receptor (BDKRB2), also known as B2R, BK-2, or BKR2, is a G protein-coupled receptor encoded by the BDKRB2 gene on human chromosome 14.
• It functions as the primary receptor for bradykinin, a potent vasoactive peptide, mediating signaling through G proteins that activate a phosphatidylinositol-calcium second messenger system.
• The receptor localizes primarily to the cell membrane, consistent with its role as a transmembrane transducer.
• Tissue expression is ubiquitous, with notable presence in normal smooth muscle tissue and neurons, reflecting its involvement in vascular regulation and nociception.
• BDKRB2 is subject to alternative splicing, producing multiple isoforms, and post-translational modifications including glycosylation, phosphorylation, and palmitoylation, which may regulate receptor activity and trafficking.
• The protein contains disulfide bonds and palmitoylation sites, contributing to its structural stability and membrane association.
• As a member of the GPCR family, BDKRB2 is a key target in cardiovascular and inflammatory disease research.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 302–391) of human BDKRB2, which may aid in detecting full-length and certain splice variants; consider sequence alignment to confirm isoform specificity.
• For Western blotting, a band around 44 kDa is expected; however, post-translational modifications or alternative splicing may yield additional bands—validate in the relevant sample system.
• In immunofluorescence/ICC, membrane localization should be prominent; appropriate fixation and permeabilization conditions are critical.
• This polyclonal antibody is suitable for ELISA applications; for sandwich ELISA development, pairing with a validated monoclonal capture antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams a unified sourcing platform for IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For BDKRB2 and other GPCR targets, we supply high-quality polyclonal and monoclonal antibodies, supporting your development from concept to clinic. Whether you need rare target sourcing or technical consulting, CamelBio streamlines your path to reliable immunoassay components.