Acetyl-Histone H3 (K36) Rabbit Polyclonal Antibody (Q16695/P68431) for WB and ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin, thus playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is dynamically regulated by post-translational modifications (PTMs), commonly referred to as the "histone code". Related references: UniProtKB P68431

Acetylation at lysine 36 (K36, corresponding to position 37 in the mature H3 protein) is a well-characterized modification associated with actively transcribed chromatin. This acetylation mark is dynamically deposited by histone acetyltransferases and removed by deacetylases, contributing to the regulation of gene expression and chromatin structure. Related references: PMID:17189264, PMID:17194708

Canonical histone H3.1 (encoded by multiple genes such as H3C1–H3C12) is expressed ubiquitously, while the testis-specific variant H3.1t (H3-4) is restricted to testicular cells. The antibody recognizes acetylated K36 across multiple H3 variants due to sequence conservation around the modification site. Related references: UniProtKB Q16695

Subcellular localization of histone H3 is nuclear, tightly associated with chromosomal DNA throughout the cell cycle. Phosphorylated H3 is a widely used marker of chromosome condensation during mitosis. Related references: UniProtKB P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide spanning K36 of human histone H3. To confirm antibody specificity, consider performing peptide blocking assays using the acetylated versus non-acetylated peptide.
• In Western blot (WB) applications, validate the antibody in relevant sample systems (e.g., HeLa whole cell lysates) and use proper controls such as histones extracted from cells treated with HDAC inhibitors to enrich acetylation.
• For ELISA, the acetylated peptide can be used as the coating antigen; activity should be verified against the unmodified peptide to ensure modification-specific binding.
• This antibody shows broad species cross-reactivity (Human, Mouse, Rat, and predicted wide range), but it is advisable to initially verify reactivity in the specific species and tissue of interest.
• Due to the polyclonal nature, lot-to-lot consistency should be monitored; include a positive control standard in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic procurement partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of raw materials spanning the entire diagnostic development pipeline. In the field of epigenetic and chromatin research, we provide access to high-affinity antibodies like this acetyl-histone H3-K36 rabbit pAb, along with optimized matched antibody pairs, bulk ancillary reagents, and rare antigen sourcing. Whatever your raw-material needs—from discovery to clinical application—CamelBio is your dedicated one-stop bridge to reliable, scalable IVD supply.