Anti-Phospho-BRD4-S484/S488 Rabbit Polyclonal Antibody for WB, DB, ELISA - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• Chromatin reader that recognizes acetylated histones, playing a key role in epigenetic memory and transcription regulation. Related references: PMID:20871596, PMID:23086925, PMID:23317504
• Remains associated with acetylated chromatin throughout the cell cycle to preserve chromatin status and enable postmitotic G1 gene transcription. Related references: PMID:22334664, PMID:23317504, PMID:23589332
• Recruits the P-TEFb complex to promoters and enhancers, collaborating with JMJD6 to activate RNA polymerase II phosphorylation. Related references: PMID:16109376, PMID:16109377, PMID:19596240
• Recognizes acetylated RELA to recruit P-TEFb and activate NF-kappa-B transcription. Related references: PMID:19103749
• Phosphorylated by CK2 and recruited to p53 target promoters, regulating p53-mediated transcription. Related references: PMID:23317504
• Acts as a chromatin insulator in the DNA damage response by recruiting the condensin-2 complex to limit H2AX phosphorylation spreading.
• Localizes predominantly in the nucleus and on chromosomes.
• Ubiquitously expressed and modified by acetylation, phosphorylation, and ubiquitination; contains conserved bromodomains.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser484 and Ser488 of human BRD4, making it suitable for detecting phosphorylation at these sites.
• For WB, consider blocking with the non-phosphorylated peptide to confirm phospho-specificity.
• The antibody is applicable in WB, DB, and ELISA; for DB and ELISA, appropriate controls should be included to validate phospho-specific binding.
• Given the large molecular weight of BRD4 (~152 kDa), ensure sufficient gel separation and transfer conditions for high-molecular-weight proteins.
• Validate the antibody’s reactivity in your specific sample system, as post-translational modifications may vary between cell types and conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. For chromatin biology and oncology applications, we offer optimized monoclonal and polyclonal antibodies and bulk sourcing options for targets like phosphorylated BRD4. Our supply chain supports every stage from early development to clinical validation, ensuring reliable, high-quality raw materials for your assay needs.