Acetyl-Histone H4 (K5/K8/K12/K16) Rabbit Polyclonal Antibody P62805 for WB, IHC-P, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, responsible for wrapping and compacting DNA into chromatin. This restricts DNA accessibility, playing a central role in fundamental cellular processes including transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt P62805
• The function of histones is critically regulated by post-translational modifications (PTMs), collectively termed the "histone code". Acetylation of specific lysine residues on histone H4, including K5, K8, K12, and K16 (positions 6, 9, 13, and 17 in the mature protein), is associated with chromatin relaxation and active gene transcription. This antibody specifically recognizes the acetylated forms at these sites. Related references: PMID:2474456; PMID:7664735; PMID:19608861
• Histone H4 is predominantly localized to the nucleus and associates with chromosomes. Upon acetylation, it remains nuclear and is enriched in transcriptionally active euchromatin regions. Related references: UniProt P62805
• The high degree of evolutionary conservation of histone H4 sequences across species underpins its predicted wide cross-reactivity with human, mouse, rat, and other organisms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide acetylated at K5, K8, K12, and K16. For best results, ensure that sample preparation retains histone acetylation marks (e.g., use of deacetylase inhibitors). Consider using non-acetylated peptide or untreated samples as specificity controls.
• For Western blot (WB), histone-enriched extracts may provide improved signal. Optimal antibody dilution should be determined empirically; typical histone H4 bands are expected at ~11 kDa.
• In immunohistochemistry (IHC-P), antigen retrieval and detection conditions may require optimization. Validate staining in positive and negative tissue types.
• For ELISA, this antibody can be used as a capture or detection reagent. Pairing with other non-competing anti-H4 antibodies may be explored for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials, from discovery to clinical application. This Acetyl-Histone H4 rabbit polyclonal antibody exemplifies our capability to supply validated, custom-ready acetylated antibody products for epigenetic and chromatin research. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to support your entire IVD workflow.