Anti-BRD4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 (Bromodomain-containing protein 4, also known as HUNK1) is a chromatin reader protein that recognizes acetylated histones, playing a key role in epigenetic memory transmission across cell divisions.
  Related references:
  PMID:20871596 PMID:23086925 PMID:23317504
• It remains associated with acetylated chromatin throughout the cell cycle to preserve chromatin status and maintain high-order structure, providing memory for postmitotic gene transcription.
  Related references:
  PMID:22334664 PMID:23317504 PMID:23589332
• During interphase, BRD4 regulates signal-inducible gene transcription by recruiting the P-TEFb complex to promoters and distal enhancers, collaborating with JMJD6 to activate P-TEFb and promote RNA polymerase II CTD phosphorylation.
  Related references:
  PMID:16109376 PMID:16109377 PMID:19596240
• BRD4 also promotes phosphorylation of 'Ser-2' of RNA polymerase II CTD, either through P-TEFb recruitment or via a reported intrinsic kinase activity that requires further in vivo evidence.
  Related references:
  PMID:23086925 PMID:22509028
• It recognizes acetylated RELA, enhancing NF-kappa-B transcriptional activation by recruiting the P-TEFb complex.
  Related references:
  PMID:19103749
• BRD4 functions as a chromatin insulator in the DNA damage response, recruiting the condensin-2 complex to acetylated histones and limiting H2AX phosphorylation spreading.
• Subcellularly, BRD4 localizes to the nucleus and associates with chromosomes.
• It is ubiquitously expressed across tissues and is subject to post-translational modifications including phosphorylation and acetylation; contains bromodomains, and is implicated in host-virus interactions and disease variants.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide within amino acids 100–200 of human BRD4, suggesting that the antibody may recognize an epitope in this N-terminal region.
• For Western blot (WB), the predicted molecular weight is ~152 kDa; consider using appropriate gel percentage and extended transfer times for such high-molecular-weight proteins. Validate with positive and negative controls in the relevant sample system.
• For immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate or EDTA buffer, heat-induced) may be optimized for your specific tissue type. Since BRD4 is ubiquitously expressed, include proper tissue controls.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with appropriate antibodies and validating with recombinant BRD4 protein or cell lysates is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. This anti-BRD4 monoclonal antibody is suitable for epigenetic and cancer research requiring reliable human BRD4 detection. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets—streamlining your development from concept to clinic.