Anti-BRSK1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8TDC3

Core Product Specifications and Parameters

Biological Background: BRSK1 Function and Localization

• BRSK1 (also known as SAD-B, hSAD1) is a serine/threonine-protein kinase belonging to the AMPK family, encoded by the BRSK1 gene on chromosome 19.
• It plays a key role in neuronal polarization by phosphorylating microtubule-associated proteins such as MAPT/TAU at Thr-529 and Ser-579, and by phosphorylating WEE1 at Ser-642 to downregulate its activity.
• BRSK1 regulates centrosome duplication by phosphorylating gamma-tubulin (TUBG1 and TUBG2) at Ser-131, promoting their translocation to the centrosome.
• The kinase is activated by STK11/LKB1-mediated phosphorylation and subsequently phosphorylates substrates including CDC25B, CDC25C, and RIMS1, linking it to cell cycle and synaptic vesicle regulation.
• It localizes to the cytoplasm, nucleus, centrosome, and synaptic vesicles, aligning with its roles in cell cycle, DNA damage response, and neurotransmitter release.
• BRSK1 is involved in the UV-induced DNA damage checkpoint by inhibiting CDK1 activity through phosphorylation and activation of WEE1 and inhibition of CDC25B and CDC25C.
• It is widely expressed, with highest levels in brain and testis, and protein levels remain constant throughout the cell cycle.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted band is at ~85 kDa; using a reducing gel and appropriate molecular weight markers is recommended.
• In immunohistochemistry (IHC-P), signal may be influenced by antigen retrieval method; testing both citrate- and EDTA-based buffers could be beneficial.
• Since the antibody is polyclonal, lot-to-lot consistency should be verified for critical quantitative assays.
• The immunogen corresponds to a region within the kinase domain and C-terminal; thus, the antibody may recognize both full-length and splice variants if the epitope is preserved.
• Cross-reactivity with mouse and rat is indicated; however, sequence homology in the immunogen region should be checked if using non-human samples.
• For ELISA applications, pairing with a BRSK1-specific capture antibody may enhance sensitivity; consider testing direct and sandwich ELISA formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For BRSK1-related studies in neurobiology and cell cycle research, CamelBio offers a reliable supply chain and technical support to accelerate your assay development.