Acetyl-Histone H3-K4 Rabbit pAb (Q16695 / P68431) for WB, IF, ELISA, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H3 Acetylation at Lysine 4 (H3K4ac)

• Histone H3 is a core component of the nucleosome, essential for packaging DNA into chromatin and regulating DNA accessibility. This central role impacts transcription, DNA repair, replication, and chromosomal stability. Related references: UniProt Q16695 and UniProt P68431

• Localization: Histone H3 is predominantly found in the nucleus and associated with chromosomes.

• The N-terminal tail of H3 undergoes extensive post-translational modifications, forming the histone code. Acetylation at lysine 4 (corresponding to K5 in the UniProt canonical sequence of P68431) is a dynamic mark linked to active chromatin states and transcriptional activation. Related references: PMID:17194708

• Tissue expression: The canonical H3.1 (P68431) is ubiquitously expressed, while the H3.1t variant (Q16695) is specific to testicular cells.

• The wide predicted cross-reactivity of this polyclonal antibody suggests conservation of the acetylated epitope across mammals, making it a valuable tool for epigenetic studies in multiple species.

Experimental Guidance and Technical Tips

• Western Blot (WB): For best results, consider using a histone extraction protocol to enrich for nuclear proteins. The predicted molecular weight is ~15 kDa; ensure adequate transfer conditions for low-molecular-weight proteins.
• IF/ICC: Methanol fixation may be suitable; however, validate the protocol in the relevant cell or tissue type.
• ELISA: Use the synthetic acetylated peptide as a positive control to confirm specificity for the acetylated epitope.
• ChIP: Peptide competition assays can help confirm specificity for acetyl-H3K4. Cross-linked chromatin preparation should be optimized for the target genomic regions.
• The antibody's wide predicted cross-reactivity suggests utility in non-human mammalian models, but validation in the specific sample system is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD manufacturers and research labs, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials. For epigenetic research, we supply a comprehensive portfolio of acetyl- and methyl-specific antibodies to support diagnostic assay development and biomarker discovery. Our team is ready to assist you in moving your project from concept to clinic.