Anti-RAB23 Rabbit Polyclonal Antibody for WB, ELISA - Q9ULC3

Core Product Specifications and Parameters

Biological Background: RAB23 Function and Localization

• Ras-related protein Rab-23 is a small GTPase that acts as a molecular switch cycling between inactive GDP-bound and active GTP-bound states, regulating intracellular membrane trafficking from vesicle formation to fusion.
• Plays a critical role in the formation and elongation of neuronal primary cilia, impacting sonic hedgehog (Shh) signaling (PubMed:40825043). Related references: PMID:40825043
• In conjunction with IFT57 and KIF17, drives the localization of specific G protein-coupled receptors, such as dopamine receptor DRD1, to primary cilia (PubMed:26182404). Related references: PMID:26182404
• Cooperates with SUFU to prevent nuclear import of GLI1 transcription factor, downregulating Shh signaling (PubMed:22365972) (PubMed:39615683). Related references: PMID:22365972, PMID:39615683
• Regulates GLI1 in differentiating chondrocytes and modulates GLI2/GLI3 transcription factor activity, including GLI3 proteolytic processing.
• Mediates defense against pathogens like S. aureus by promoting capture by autophagosomes that merge with lysosomes (PubMed:22452336). Related references: PMID:22452336
• Subcellularly localized at the cell membrane, cytoplasm, and various vesicle membranes including endosomes, autophagosomes, and phagosomes.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length RAB23 protein (amino acids 1-237), which may detect total RAB23 levels; consider using appropriate positive controls (e.g., cell lines with high endogenous RAB23 expression) in Western blotting.
• For ELISA applications, optimization of antibody coating concentration and detection range should be validated in the relevant sample system.
• Given the antibody's species cross-reactivity with human, mouse, and rat, it may be suitable for comparative studies; confirm reactivity in your specific model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. For studies on ciliary trafficking and hedgehog signaling, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-RAB23 pAb, and bulk ancillary reagents. Our supply network also covers rare target raw materials, ensuring consistency for your assay development.