BRD4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 is a bromodomain-containing protein that functions as a chromatin reader, specifically recognizing and binding acetylated histones to transmit epigenetic memory across cell divisions and maintain high-order chromatin structure. Related references: PMID:20871596 PMID:23086925 PMID:23317504 PMID:22334664 PMID:23589332

• It plays a critical role in transcription regulation by associating with the P-TEFb complex and recruiting it to promoters of signal-inducible genes, thereby promoting RNA polymerase II elongation. Related references: PMID:16109376 PMID:16109377 PMID:19596240

• BRD4 also targets distal enhancers, where it collaborates with JMJD6 to form an active P-TEFb complex by displacing negative regulators like HEXIM1 and 7SK snRNA. Related references: PMID:16109376 PMID:16109377 PMID:19596240

• The protein directly or indirectly mediates phosphorylation of the C-terminal domain (CTD) of RNA polymerase II at serine-2, a key step in transcription elongation. Related references: PMID:23086925 PMID:22509028

• Beyond histones, BRD4 recognizes acetylated RELA, facilitating NF-kappa-B-mediated transcriptional activation. Related references: PMID:19103749

• BRD4 is also recruited to p53/TP53 target promoters after CK2 phosphorylation, acting as a regulator of p53-dependent transcription. Related references: PMID:23317504

• In the DNA damage response, BRD4 acts as a chromatin insulator by recruiting the condensin-2 complex to acetylated histones, thereby limiting H2AX phosphorylation spreading.

• Subcellularly, BRD4 localizes to the nucleus and chromatin; it is ubiquitously expressed across tissues, reflecting its fundamental role in chromatin biology and transcription.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 700-794 of human BRD4) targets a region outside the conserved bromodomains, potentially offering specificity for BRD4 over other BET family members; however, cross-reactivity should be confirmed in the relevant experimental system.
• For Western blot applications, an expected band around 152 kDa should be detected; optimize antibody dilution and exposure times to achieve clear results.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), BRD4 nuclear staining is anticipated; ensure proper fixation and antigen retrieval methods to preserve epitope accessibility.
• When using this antibody in ELISA, consider using the immunogen peptide as a positive control and validate performance across different sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by providing high-quality reagents like this anti-BRD4 polyclonal antibody, supporting research into epigenetic regulation and transcriptional control. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic assay development. With a global sourcing network, we also specialize in rare and custom target raw materials, bridging your projects from concept to clinic.