Acetyl-Histone H3-K36 (Q16695/P68431) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, essential for packaging DNA into chromatin and regulating fundamental processes like transcription, DNA repair, and replication.
Related references: UniProtKB Q16695, UniProtKB P68431

The "histone code" hypothesis posits that post-translational modifications (PTMs) on histones — including acetylation, methylation, and phosphorylation — serve as recognition platforms for chromatin-binding proteins, thereby modulating chromatin architecture and gene expression. Acetylation at specific lysine residues, such as K36, is associated with active transcription and open chromatin.
Related references: PMID:19608861, PMID:17194708

The antibody’s immunogen is a synthetic peptide carrying an acetyl group at lysine 36 of human Histone H3. It recognizes the acetylated form of H3K36 in multiple variants, including the canonical H3.1 (P68431) and the testis-specific H3.1t (Q16695). While H3.1 is ubiquitously expressed, H3.1t shows enriched expression in testicular cells.
Related references: UniProtKB Q16695

Histone H3 localizes exclusively to the nucleus and is an integral part of chromosomes, with its N‑terminal tail protruding from the nucleosome core to undergo dynamic modifications.
Related references: UniProtKB P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide covering residues around K36 of human Histone H3. For detection of endogenous acetyl‑H3K36, consider that the modification may be cell‑cycle or treatment‑dependent; histone extraction or acid‑extraction protocols may improve signal.
• In Western blot (WB), a major band at approximately 15 kDa is expected. Because histones are highly conserved, the antibody is predicted to cross‑react with a wide range of species; nonetheless, validation in the relevant sample system is recommended.
• For immunofluorescence (IF/ICC), fixation and permeabilization steps should be carefully optimized, as epitope accessibility can be influenced by chromatin compaction.
• ELISA‑based detection can be used for quantitative analysis of acetyl‑H3K36; titrate the antibody and confirm the coating concentration of the acetylated peptide.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research teams with a comprehensive portfolio of raw materials and sourcing solutions. For epigenetic and chromatin biology applications, this acetyl‑Histone H3‑K36 rabbit polyclonal antibody provides a reliable tool to interrogate histone modification states. Beyond individual reagents, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, enabling streamlined procurement from early‑stage development through scale‑up.