[KO Validated] Anti-BRD7 Rabbit Polyclonal Antibody for WB, ELISA - Q9NPI1

Core Product Specifications and Parameters

Biological Background: BRD7 Function and Localization

• BRD7 (Bromodomain-containing protein 7), also known as BP75, NAG4, or CELTIX-1, is a 75 kDa nuclear protein that contains a bromodomain and coiled-coil region, acting as both a transcriptional coactivator and corepressor.
• It participates in chromatin remodeling and modulates gene expression by binding to target promoters, increasing histone H3 acetylation at Lys-9 (H3K9ac).
• BRD7 activates the Wnt signaling pathway by negatively regulating GSK3B activity, leading to dephosphorylation at Tyr-216 in a DVL1-dependent manner.
• As a coactivator for TP53, BRD7 promotes TP53 acetylation at Lys-382, facilitates TP53 recruitment to target promoters, and is required for TP53-mediated cell-cycle arrest upon oncogene activation.
• It also acts as a corepressor, down-regulating transcription of specific genes, and recruits BRCA1 and POU2F1 to the ESR1 (estrogen receptor) promoter.
• BRD7 inhibits cell cycle progression from G1 to S phase, consistent with its tumor suppressor activity.
• Subcellularly, BRD7 is localized to the nucleus and associated with chromosomes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination (isopeptide bond), with keywords such as alternative splicing, tumor suppressor, and Wnt signaling pathway.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein corresponding to amino acids 1-95 of human BRD7 (NP_037395.2). Consider that it may preferentially recognize the N-terminal region and validate for isoform specificity if needed.
• For Western blot, we recommend starting with a standard dilution range (e.g., 1:500–1:2000) and optimizing based on your sample system; the expected molecular weight is ~74 kDa.
• For ELISA, titrate the antibody against the immunogen or target protein to determine optimal coating and detection concentrations.
• As a polyclonal antibody, batch-to-batch consistency should be validated for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies for targets like BRD7 involved in cancer and signaling research. Our comprehensive portfolio also covers bulk ancillary reagents and rare target raw-material sourcing, enabling seamless integration from R&D to clinical application. With expert technical support and supply chain efficiency, CamelBio bridges the gap between concept and clinic for your diagnostic development needs.