Acetyl-Histone H4-K5 Rabbit Polyclonal Antibody (P62805) – Validated for WB, IHC-P, ELISA, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, responsible for wrapping and compacting DNA into chromatin. This compaction restricts DNA accessibility, thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt P62805

• The protein localizes primarily to the nucleus and associates with chromosomes. It has been observed to be localized to the nucleus when acetylated in step 11 spermatids. Related references: UniProt P62805

• Histone H4 undergoes extensive post-translational modifications (PTMs) on its N-terminal tail, which together constitute the "histone code." Acetylation at lysine 5 (the target of this antibody) is a key modification associated with active chromatin states. Related references: PMID:2474456, PMID:7664735, PMID:19608861

• Additional modifications include methylation, phosphorylation, and various acylations, which coordinate to regulate gene expression and other DNA-templated processes. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide spanning amino acids 1-100 of human Histone H4, which includes the N-terminal tail and the acetylated Lys5 epitope. Consider using non-acetylated peptide or mutant (K5A) as a negative control to confirm specificity.
• Western Blot (WB): Histone H4 has a predicted molecular weight of ~11 kDa. Ensure adequate separation on high-percentage SDS-PAGE gels (e.g., 15-18%) and verify band intensity normalized to total histone loading controls (e.g., Histone H3, total H4).
• Immunohistochemistry-Paraffin (IHC-P): Antigen retrieval is recommended due to the compact nature of chromatin; citrate buffer (pH 6.0) or Tris-EDTA (pH 9.0) may be tested. Acetylation patterns may vary by tissue and fixation conditions.
• ELISA: The antibody can be used to detect acetylated histone peptides or acid-extracted histones coated directly on plates. Optimize coating concentration and blocking steps to minimize background.
• Chromatin Immunoprecipitation (ChIP): Suitable for genome-wide mapping of H4K5ac loci. Cross-linking time and chromatin shearing conditions should be empirically determined for each sample type. A post-ChIP Western blot is advised to confirm enrichment.
• Cross-reactivity: The immunogen sequence is highly conserved across human, mouse, and rat, and the manufacturer predicts a wide range of reactivity. Validate in your species of interest by sequence alignment and preliminary testing.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as a comprehensive sourcing partner for IVD manufacturers and research institutions, providing one-stop access to raw materials, technical services, and consulting from concept to clinic. This Acetyl-Histone H4-K5 Rabbit pAb is part of our curated portfolio of acetylated antibodies, supporting epigenetics and chromatin research. Beyond this product, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic and research workflows. Contact us to explore tailored solutions for your project.