Acetyl-Histone H4-K5 Rabbit Polyclonal Antibody (P62805) – WB, ELISA, ChIP Applications

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This limits DNA accessibility to the cellular machineries that require DNA as a template, placing histones at the center of transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: none directly cited; general functional annotation.

• The DNA accessibility is modulated through a complex set of post-translational modifications (PTMs) known as the "histone code," along with nucleosome remodeling. One critical PTM is the acetylation of lysine 5 (K5), which corresponds to N6‑acetyllysine at position 6 of the canonical UniProt sequence. Related references: PMID:2474456, PMID:7664735, PMID:19608861.

• Subcellular localization is predominantly nuclear, with association to chromosomes. During spermatogenesis, acetylated Histone H4 has been observed to localize specifically to the nucleus in step 11 spermatids. Related references: (By similarity) UniProtKB P62805.

• Histone H4 undergoes numerous other modifications, including methylation, citrullination, crotonylation, phosphorylation, and ubiquitination, which collectively orchestrate gene expression patterns and chromatin dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide spanning the K5 region of human Histone H4. This design aims to confer high specificity for the acetylated form of the protein.
• For Western blot (WB), consider using nuclear‑enriched extracts or acid‑extracted histones to improve detection. A band around 11 kDa is expected.
• In ELISA, the antibody may be used to capture or detect acetylated histone peptides or recombinant Histone H4 proteins. Titration is recommended for optimal signal‑to‑noise.
• For chromatin immunoprecipitation (ChIP), this antibody can be considered for enriching genomic regions marked by H4K5ac. Validation in the relevant cell type and appropriate controls (e.g., non‑immune IgG, input chromatin) is essential.
• Due to the high conservation of the histone H4 N‑terminus, wide cross‑reactivity is predicted among human, mouse, rat, and potentially other species. However, experimental verification in the target species is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a one‑stop resource for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This Acetyl-Histone H4-K5 rabbit polyclonal antibody complements a broad epigenetics portfolio, and our team can further support your project with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing. Partner with CamelBio to streamline your assay development and secure reliable, research‑grade materials for chromatin and epigenetic applications.