BRD7 Rabbit pAb - Q9NPI1

Core Product Specifications and Parameters

Biological Background: BRD7 Function and Localization

• Alternative names: 75 kDa bromodomain protein, Protein CELTIX-1, and also known as BP75, NAG4, SMARCI1.
• Functions as both transcriptional coactivator and corepressor, and may play a role in chromatin remodeling.
• Activates the Wnt signaling pathway by negatively regulating GSK3B kinase activity (dephosphorylation at Tyr-216) in a DVL1-dependent manner.
• Acts as a corepressor by binding target promoters, increasing histone H3 acetylation at Lys-9 (H3K9ac), and downregulating gene expression (e.g., ESR1 promoter).
• Coactivates TP53-mediated transcription, promoting TP53 acetylation at Lys-382 and efficient recruitment to promoters; required for TP53-mediated cell-cycle arrest.
• Inhibits cell cycle progression from G1 to S phase, consistent with tumor suppressor activity.
• Subcellular localization: predominantly nuclear, associated with chromosomes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (isopeptide bond).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–95) of human BRD7; consider potential recognition of isoforms sharing this epitope.
• For Western blot, expect a band near 74 kDa; validate using whole-cell lysates from human, mouse, or rat cell lines. Polyclonal antibodies may require titration for optimal signal-to-noise ratio.
• For ELISA, use recombinant protein or cell lysates; optimize coating conditions and antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated B2B partner for IVD raw materials, offering validated antibodies, ancillary reagents, and custom sourcing for challenging targets. This BRD7 antibody aligns with research areas such as transcription, chromatin biology, and tumor suppression—areas where we supply optimized polyclonal and monoclonal reagents, bulk ancillary materials, and rare target sourcing to support your diagnostic and research workflows from concept to clinic.