Acetyl-alpha Tubulin (K40) Rabbit pAb (Q71U36) for WB, IF-P, ELISA

Core Product Specifications and Parameters

Biological Background: Tubulin alpha-1A Chain Function and Localization

Tubulin alpha-1A chain (TUBA1A, also known as TUBA3, B-ALPHA-1) is a major constituent of microtubules. Microtubules are dynamic cylindrical polymers composed of alpha- and beta-tubulin heterodimers that assemble into linear protofilaments. They grow by the addition of GTP-tubulin dimers at the plus end, forming a stabilizing cap; below the cap, GDP-bound dimers predominate due to the GTPase activity of alpha-tubulin. This dynamic instability is essential for cell division, intracellular transport, and structural support.

• Subcellular localization: Predominantly cytoplasmic, incorporated into the cytoskeleton. In specialized cell types, it is also found in the axoneme of flagella. Related references: UniProtKB Q71U36

• Tissue specificity: Highly expressed in fetal brain, reflecting its critical role in neurodevelopment. Related references: PMID:17584854

• Post-translational modifications: Lysine 40 (K40) is a major acetylation site. Acetylation at this residue stabilizes microtubules and is a hallmark of long-lived, stable microtubule populations. Other modifications include phosphorylation, polyglutamylation, and nitration. Related references: PMID:24906155, PMID:32747782, PMID:10339593

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around K40; thus, the antibody is expected to specifically recognize the acetylated form of alpha-tubulin. For detection of endogenous acetylation, consider using deacetylase inhibitors (e.g., trichostatin A) in your experimental system to preserve the modification.
• Validated applications include WB, IF-P, and ELISA. For WB, a band around 50 kDa is expected. Due to high conservation, cross-reactivity with mouse and rat samples has been confirmed; however, validation in your specific model system is recommended.
• In immunofluorescence (IF-P), ensure appropriate fixation and permeabilization to preserve microtubule structures. Co-staining with a total alpha-tubulin antibody can help assess acetylation levels.
• As with any polyclonal antibody, lot-to-lot consistency may vary; include positive and negative controls (e.g., untreated vs. deacetylase-treated cells) in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This acetyl-alpha tubulin (K40) rabbit polyclonal antibody addresses the growing need for high-quality immuno-reagents in microtubule biology, neurodevelopment, and cancer research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. Contact us to explore flexible procurement solutions tailored to your project requirements.