[KO Validated] Anti-βIII-Tubulin Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q13509

Core Product Specifications and Parameters

Biological Background: βIII-Tubulin Function and Localization

• Tubulin beta-3 chain (TUBB3), also designated beta-4 or beta-III tubulin, is a major microtubule constituent.
• Tubulin heterodimers of alpha- and beta‑tubulin polymerize into microtubules. TUBB3 is critical for proper axon guidance and maintenance, and mediates netrin‑1/UNC5C‑induced axon repulsion. Related references: PMID:34996871, PMID:38305685, PMID:38609661, PMID:20074521, PMID:28483977
• Subcellularly, it localizes to the cytoplasm and cytoskeleton, with enrichment in neuronal growth cones, lamellipodia, and filopodia.
• Expression is primarily restricted to the central and peripheral nervous systems and is strongly upregulated in most cancerous tissues.
• Known post‑translational modifications include phosphorylation and isopeptide bond formation.
• Mutations in TUBB3 are linked to neurological disorders such as congenital fibrosis of the extraocular muscles (CFEOM), cortical dysplasia, and other brain malformations.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 40–260 of human βIII‑tubulin; verify coverage of your target isoform if working with non‑human or variant sequences.
• For western blotting, a band at ~50 kDa is expected; validate loading controls and consider the endogenous expression profile in your sample.
• In immunofluorescence, optimize permeabilization and fixation to preserve microtubule structure; counterstain with a nuclear marker to reveal the cytoplasmic distribution.
• When using ELISA, pair with an appropriate detection antibody and confirm cross‑reactivity against mouse or rat antigens if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive support for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For neuroscientific and oncological research, we help secure rare‑target materials like TUBB3 antibodies, enabling seamless development from concept to clinic.