Anti-Adenylate Kinase 4 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27144

Core Product Specifications and Parameters

Biological Background: Adenylate kinase 4 Function and Localization

• Adenylate kinase 4 (AK4) is a broad-specificity mitochondrial nucleoside phosphate kinase that maintains cellular nucleotide homeostasis by catalyzing nucleoside-phosphate interconversions. Related references: PMID:19073142 PMID:19766732 PMID:23416111
• AK4 preferentially phosphorylates AMP to ADP using ATP as the phosphate donor; when using GTP, it exclusively phosphorylates AMP. Related references: PMID:19766732
• In vitro, AK4 can also phosphorylate CMP, dAMP, and dCMP and use GTP as an alternate phosphate donor. Related references: PMID:19766732 PMID:23416111
• AK4 exhibits diphosphate kinase activity, generating various nucleoside triphosphates (ATP, CTP, GTP, UTP, TTP, dATP, dCTP, dGTP) from their diphosphate substrates. Related references: PMID:23416111
• AK4 is localized to the mitochondrial matrix.
• AK4 shows high expression in kidney, moderate in heart and liver, and low in brain.
• AK4 protects cells from oxidative stress and modulates cellular energy sensing by controlling AMPK phosphorylation. Related references: PMID:19130895 PMID:23474458 PMID:24767988 PMID:26980435

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering amino acids 1–100 of human AK4, an N-terminal region likely exposed in the native protein; this design supports applications like IF/ICC where native conformation may be critical.
• For western blot (WB), use reducing conditions to resolve the protein at ~25 kDa. Consider validating specificity in both human and rat lysates, as cross-reactivity with rat is reported.
• Optimization of antibody dilution, antigen retrieval, and blocking conditions is recommended for IHC-P and IF/ICC, as performance may vary with sample type and fixation.
• In ELISA, titrate coating antigen and detection antibodies to achieve optimal signal-to-noise ratios; the antibody’s reactivity with a linear peptide immunogen suggests suitability for both direct and sandwich ELISA formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers, laboratories, and research institutes by providing reliable access to validated raw materials such as this AK4 rabbit monoclonal antibody, ideal for exploring mitochondrial nucleotide metabolism, AMPK signaling, and oxidative stress pathways. Our comprehensive portfolio includes optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring seamless integration into your diagnostic development workflow from concept to clinical application.