Anti-Tubulin beta-1 chain Monoclonal Antibody for WB, IF/ICC, ELISA - Q9H4B7

Core Product Specifications and Parameters

Biological Background: Tubulin beta-1 chain Function and Localization

• Tubulin beta-1 chain, encoded by the TUBB1 gene, is a beta-tubulin isotype that polymerizes with alpha-tubulin to form microtubules, the major constituent of the cytoskeleton.
• Microtubules grow through the addition of GTP-bound tubulin dimers; GTP hydrolysis to GDP weakens lateral interactions, promoting depolymerization and dynamic instability.
• TUBB1 is specifically expressed in hematopoietic cells and is the predominant beta-tubulin in leukocytes, constituting about 50% of total beta-tubulin in these cells.
• The protein is primarily localized in the cytoplasm and cytoskeleton, integrating into the microtubule network.
• Key post-translational modifications include phosphorylation and isopeptide bond formation, which may regulate microtubule stability and interactions.
• Mutations in the TUBB1 gene have been linked to certain blood cell disorders, highlighting its importance in hematopoietic function.
• The mature protein has a calculated molecular mass of approximately 50.3 kDa, consistent with the observed 50 kDa band.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 352-451) of human Tubulin beta-1 chain, which may enable detection of specific isoforms or post-translationally modified forms.
• For western blot, a band at ~50 kDa is expected; optimal dilution and blocking conditions should be determined empirically using relevant hematopoietic cell lysates (e.g., Jurkat, THP-1).
• In IF/ICC, microtubule staining patterns can be visualized; fixation and permeabilization protocols should be optimized to preserve filament integrity.
• Cross-reactivity with mouse, rat, cow, dog, and monkey has been reported; validate antibody performance in the target species sample type before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for diagnostic manufacturers and research laboratories working on cytoskeleton and microtubule biology. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your IVD development from concept to clinic. For rare target or custom raw-material needs, our team ensures reliable supply-chain integration.