Anti-α-Actinin-4 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - O43707

Core Product Specifications and Parameters

Biological Background: α-Actinin-4 Function and Localization

• Encoded by the ACTN4 gene, alpha-actinin-4 is a non-muscle actin-bundling protein that cross-links F-actin and anchors it to intracellular structures.
• Functions as a transcriptional coactivator by stimulating PPARG and RARA-mediated transcription. Related references: PMID:22351778
• Essential for transferrin receptor recycling through association with the CART complex, necessary for efficient endocytic trafficking. Related references: PMID:15772161
• Facilitates tight junction assembly in epithelial cells by linking MICALL2 to the actin cytoskeleton and recruiting it to tight junctions.
• Regulates immune synapse formation and efficient T-cell activation via association with IGSF8. Related references: PMID:22689882
• Localizes to diverse subcellular compartments including the nucleus, cytoplasm, cell junctions, stress fibers, and perinuclear region.
• Post-translational modifications such as acetylation and phosphorylation, along with alternative splicing, regulate its functions; it also exhibits calcium and metal ion binding.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human α-Actinin-4; consider epitope accessibility in different experimental conditions.
• For Western blotting, the predicted molecular weight is approximately 105 kDa; verify band specificity using appropriate positive and negative controls.
• In IF/ICC, α-Actinin-4 displays nuclear, cytoplasmic, and junctional staining; optimize fixation and permeabilization steps to preserve antigenicity and subcellular localization.
• Cross-reactivity with mouse and rat orthologs enables comparative studies across species, but validate in the relevant sample system.
• This antibody is declared for WB, IF/ICC, IP, and ELISA; performance in other applications should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, technical services, and consulting. This anti-α-Actinin-4 antibody supports research in actin cytoskeleton dynamics, cell adhesion, and nuclear transcription. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized rare-target raw-material sourcing to accelerate your project from concept to clinic.