Anti-AKT1S1/PRAS40 Rabbit Polyclonal Antibody for WB, ELISA - Q96B36

Core Product Specifications and Parameters

Biological Background: AKT1S1/PRAS40 Function and Localization

• AKT1S1/PRAS40, also designated 40 kDa proline-rich AKT substrate, is a negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), a central nutrient sensor driving anabolic cell growth. Related references: PMID:17277771, PMID:17386266, PMID:17510057
• Under nutrient-poor conditions, AKT1S1 associates with mTORC1 and directly inhibits its activity by blocking substrate recruitment. Related references: PMID:29236692
• Insulin and nutrient signaling lead to AKT1S1 phosphorylation, causing its dissociation from mTORC1 and binding to 14-3-3 proteins, thereby relieving mTORC1 inhibition. Related references: PMID:17386266, PMID:18372248, PMID:16174443
• AKT1S1 is a phosphoprotein subject to methylation; alternative splicing yields multiple isoforms, and its 3D structure has been solved.
• The protein localizes to the cytoplasm and cytosol.
• AKT1S1 is expressed widely, with highest levels in liver and heart, and is upregulated in certain cancer cell lines (e.g., A-549, HeLa) compared to normal cell lines (e.g., HEK293).

Experimental Guidance and Technical Tips

• The immunogen (aa 150–256) targets a central region of AKT1S1; performance may vary across splice variants—validate in relevant human cell models.
• For Western blot, a band near 27 kDa is expected; consider using human liver or heart lysate as positive control, given high expression in these tissues.
• Phosphorylation-dependent mobility shifts may occur; treating samples with phosphatase could confirm band identity.
• For ELISA, perform checkerboard titrations to optimize coating antigen and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, connecting you with critical reagents for mTOR signaling and nutrient sensing research. In addition to this AKT1S1/PRAS40 polyclonal antibody, we supply validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, and can assist in sourcing rare-target raw materials to support your entire assay development pipeline.