Anti-α-Tubulin Rabbit Monoclonal Antibody for WB - P68366

Core Product Specifications and Parameters

Biological Background: α-Tubulin Function and Localization

• Tubulin alpha-4A chain (TUBA4A), also known as alpha-tubulin 1 or testis-specific alpha-tubulin, is an isotype of the alpha-tubulin family, a core component of microtubules.
• Alpha-tubulin forms heterodimers with beta-tubulin, which polymerize into linear protofilaments that assemble into the hollow cylinders of microtubules, major components of the cytoskeleton.
• Microtubule dynamics are regulated by GTP binding and hydrolysis; GTP-bound tubulin adds to growing ends, while GTP hydrolysis to GDP in the protofilament lattice promotes depolymerization.
• TUBA4A localizes predominantly to the cytoplasm and the cytoskeleton, supporting intracellular transport, cell division, and maintenance of cell shape.
• This protein is subject to various post-translational modifications, including acetylation, phosphorylation, methylation, and nitration, which modulate microtubule stability and interactions.
• Expression of TUBA4A is enriched in testis, and alternative splicing generates multiple isoforms, contributing to tissue-specific microtubule functions.
• Mutations in TUBA4A have been linked to amyotrophic lateral sclerosis (ALS), a neurodegenerative disorder, highlighting its role in neuronal health.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C-terminal region (amino acids 349–448) of human α-tubulin, a conserved sequence that likely explains the cross-reactivity with mouse and rat.
• For western blotting, expect a dominant band at approximately 50 kDa; validate the signal in relevant tissue or cell lysates with appropriate positive and negative controls.
• In immunofluorescence and immunohistochemistry, optimize fixation and permeabilization to preserve microtubule structures; methanol fixation may be suitable for cytoskeletal targets.
• For immunoprecipitation, confirm compatibility of the rabbit monoclonal antibody with your experimental set-up, considering isotype and binding affinity.
• When applying the antibody to ELISA or other immunoassays, titrate to determine optimal working concentrations, as no standardized dilutions are provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with integrated access to IVD raw materials, customized technical services, and expert consulting for every stage from concept to clinic. This anti-α-tubulin antibody supports microtubule and cytoskeleton research applications, including biomarker discovery and assay development. Beyond single antibodies, we offer validated antibody pairs, optimized recombinant proteins, bulk ancillary reagents, and rare-target sourcing to accelerate your project timelines.