Acetyl-alpha Tubulin K40 Rabbit Monoclonal Antibody (Q71U36) for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Tubulin alpha-1A Chain Function and Localization

• Tubulin is the major constituent of microtubules, forming a cylindrical structure from laterally associated alpha- and beta-tubulin heterodimers. Microtubules grow by adding GTP-bound dimers to the plus end, where a stabilizing cap forms. Below the cap, GTP hydrolysis (EC 3.6.5.-) converts tubulin to a GDP-bound state, making it prone to depolymerization. This dynamic instability is fundamental to cell division, intracellular transport, and structural integrity. Related references: UniProt Q71U36.

• Alpha-tubulin localizes predominantly to the cytoplasm and cytoskeleton. It is also a component of ciliary and flagellar axonemes, where it forms the microtubular core essential for motility and signaling. Related references: UniProt Q71U36.

• Expression is particularly high in fetal brain tissue, suggesting a critical role in early neuronal development and axon formation. Related references: PMID:17584854.

• The antibody specifically recognizes alpha-tubulin acetylated at lysine 40 (K40). Acetylation at this residue is a marker of stable microtubules, especially in neurons, cilia, and flagella. Alpha-tubulin also undergoes other post-translational modifications such as polyglutamylation, phosphorylation, and nitrotyrosine, which regulate microtubule function and interactions. Related references: PMID:24906155; PMID:32747782; PMID:10339593.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic acetylated peptide around K40; therefore, the antibody is expected to detect acetylated alpha-tubulin preferentially over the non-acetylated form. Consider using acetylase inhibitors or control treatments to validate acetylation-specific signals.
• For western blotting, a major band at ~50 kDa is anticipated. Use fresh samples with protease inhibitors and avoid multiple freeze-thaw cycles to preserve labile acetylation marks.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation (e.g., 4% paraformaldehyde) to maintain epitope availability. Include isotype controls and omit-primary controls to confirm staining specificity.
• Given the high expression in fetal brain, brain tissue or neuronal cell lines may serve as positive controls. Cross-reactivity with mouse and rat enables translational and preclinical studies.
• For ELISA, coat with acetylated peptide or recombinant protein to assess binding. Pair with non-acetylated peptide as negative control to ensure epitope specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from concept to clinic. This acetyl-alpha tubulin K40 antibody facilitates research into microtubule dynamics, neurobiology, and cytoskeletal disorders. Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—ensuring you have the right tools for assay development. Reach out to CamelBio for tailored procurement solutions that accelerate your project.