Anti-Integrin alpha 4 (ITGA4/CD49d) Rabbit Polyclonal Antibody for WB, ELISA - P13612

Core Product Specifications and Parameters

Biological Background: Integrin alpha 4 (ITGA4/CD49d) Function and Localization

• Integrin alpha-4 (ITGA4/CD49d) is a transmembrane glycoprotein that pairs with integrin beta-1 or beta-7 to form functional receptors (VLA-4 or LPAM-1).
• ITGA4:ITGB1 (VLA-4) and ITGA4:ITGB7 serve as cell adhesion receptors that bind fibronectin (recognizing CS-1/CS-5 regions), VCAM1, and MADCAM1, mediating immune cell trafficking and leukocyte adhesion.
• On activated endothelial cells, VLA-4 triggers homotypic aggregation of leukocytes and may participate in cytolytic T-cell interactions.
• ITGA4:ITGB1 acts as a coreceptor for fractalkine (CX3CL1) and binds PLA2G2A, inducing conformational changes that enhance classical ligand binding (PubMed:23125415, PubMed:18635536, PubMed:25398877). Related references: PMID:23125415 PMID:18635536 PMID:25398877
• In vascular smooth muscle cells, ITGA4:ITGB1 represses vasocontraction by inhibiting Ca²⁺ influx and ROCK-mediated calcium sensitivity via interaction with SVEP1 (PubMed:35802072). Related references: PMID:35802072
• Subcellular localization is at the plasma membrane; expression is detected in vascular smooth muscle cells and leukocyte cell lines.
• Post-translational modifications include disulfide bonds, phosphorylation, and N‑linked glycosylation; the protein contains calcium‑ and metal‑binding sites, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen (residues 770–970) lies within the extracellular domain, predicted to be accessible in both native and denatured formats; consider optimizing antigen retrieval for IHC or other spatial applications.
• For Western blot, the predicted molecular weight is ~115 kDa; validate with suitable positive controls such as vascular smooth muscle cells or Jurkat T‑cell lysates.
• For ELISA, this polyclonal antibody can be evaluated as a capture or detection reagent using recombinant ITGA4 protein or cell‑derived material; always include negative controls to assess non‑specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This anti‑ITGA4 polyclonal antibody supports investigations into cell adhesion, inflammation, and immune cell migration. We offer validated antibody pairs, bulk ancillary reagents, and efficient sourcing for rare‑target raw materials to streamline your assay development from concept to clinic.