Anti-α-Actinin Monoclonal Antibody for WB, IHC-P, ELISA - P12814

Core Product Specifications and Parameters

Biological Background: α-Actinin Function and Localization

• α-Actinin-1, also known as non-muscle alpha-actinin-1 or alpha-actinin cytoskeletal isoform, is an F-actin cross-linking protein that anchors actin filaments to a variety of intracellular structures.
• It regulates immune synapse formation through association with IGSF8, which is required for efficient T-cell activation. Related references: PMID:22689882
• The protein localizes to the cytoplasm, cytoskeleton, myofibril sarcomere Z line, cell membrane, cell junctions, and cell projections such as ruffles.
• It contains actin-binding domains and calcium-binding motifs, and is subject to post-translational modifications including acetylation and phosphorylation.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• Keywords from UniProt include actin-binding, calcium, metal-binding, phosphoprotein, and disease variant, highlighting its regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 281-380 of human α-Actinin-1 (NP_001093.1), located in a conserved central region; this design may confer epitope specificity.
• For Western blotting, the expected molecular weight is approximately 103 kDa; pre-stained markers and appropriate loading controls are recommended.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval methods; validate staining in the relevant tissue type.
• In ELISA, the antibody can be used for capturing or detecting α-Actinin-1; optimal coating and detection antibody concentrations should be determined experimentally.
• Cross-reactivity with mouse suggests potential utility in mouse model studies; however, user validation for specific applications is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your α-Actinin research with reliable antibody raw materials. As a one-stop IVD sourcing partner, we provide validated monoclonal antibodies, OEM antibody pairs, and bulk ancillary reagents to streamline diagnostic development from concept to clinic. Whether you are developing cell adhesion or cytoskeleton-related assays, our supply chain can meet your specialized needs.