Anti-βIII-Tubulin Monoclonal Antibody for IF, IHC, ELISA - Q13509

Core Product Specifications and Parameters

Biological Background: βIII-Tubulin (TUBB3) Function and Localization

• βIII-Tubulin, encoded by the TUBB3 (TUBB4) gene, is a neuron-specific beta-tubulin isotype that forms heterodimers with alpha-tubulin to assemble microtubules, the major cytoskeletal filaments.
• Microtubules grow by the addition of GTP-tubulin dimers to the plus end, where a stabilizing GTP cap forms; below the cap, alpha-beta tubulin heterodimers are in GDP-bound state due to alpha-tubulin GTPase activity. Related references: PMID:34996871, PMID:38305685, PMID:38609661
• TUBB3 plays a critical role in axon guidance and maintenance; it is involved in dorsal root ganglion axon projection towards the spinal cord. Related references: PMID:20074521, PMID:28483977
• Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3, leading to increased microtubule dynamics and axon repulsion. Related references: PMID:28483977
• Subcellularly, TUBB3 localizes to the cytoplasm, cytoskeleton, and is enriched in cell projections such as growth cones, lamellipodia, and filopodia.
• Expression is primarily restricted to the central and peripheral nervous system; greatly increased expression is observed in most cancerous tissues.
• The protein features GTP-binding, magnesium binding, and undergoes phosphorylation; it is associated with disease variants linked to intellectual disability and other neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 40-260 of human TUBB3 (Q13509), a region likely exposed and conserved enough to allow cross-reactivity with mouse and rat, as indicated.
• For immunofluorescence (IF-F, IF-P), consider optimizing fixation and permeabilization conditions, as microtubule antigens may require specific protocols; validate in neuronal cell lines or primary cultures.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary; test with positive control tissues such as brain or tumor sections.
• For ELISA, the recombinant immunogen can serve as a positive control; use appropriate blocking and detection steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This βIII-Tubulin antibody exemplifies our support for neuroscience and oncology research, bridging your assay development needs from concept to clinic with reliable, high-quality materials.