βII-Tubulin/β2-Tubulin Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13885 / Q9BVA1

Core Product Specifications and Parameters

Biological Background: βII-Tubulin/β2-Tubulin Function and Localization

• βII-Tubulin (TUBB2A) and β2-Tubulin (TUBB2B) are class II beta-tubulin isotypes that heterodimerize with alpha-tubulin to form microtubules, essential cytoskeletal polymers.
• Microtubules act as dynamic tracks for intracellular transport, mitotic spindle formation, and maintenance of cell shape, growing by addition of GTP-bound tubulin dimers.
• The β-tubulin subunits contain a GTP-binding site and regulate microtubule stability through GTP hydrolysis, while alpha-tubulin provides the GTPase activity.
• βII-Tubulin is highly expressed in brain, comprising up to 30% of all beta-tubulins, and plays a critical role in neuronal development and function.
• Post-translational modifications such as acetylation, phosphorylation, and methylation modulate microtubule properties and interactions with motor proteins.
• Mutations in TUBB2A are linked to neurological disorders (e.g., cortical dysplasia, complex, with other brain malformations 5), underscoring its importance in the central nervous system.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 346-445 of human βII-Tubulin/β2-Tubulin (Q13885), a region conserved between TUBB2A and TUBB2B, making the antibody suitable for detecting both isotypes.
• For Western blotting, a band around 50 kDa is expected; optimize loading amounts (typically 10-30 µg of total protein) and antibody dilution for your specific sample.
• In immunohistochemistry (IHC-P), antigen retrieval methods using citrate or EDTA buffers may enhance signal, especially in formalin-fixed tissues.
• For immunofluorescence (IF/ICC), consider permeabilization with Triton X-100 or methanol to allow antibody access to the microtubule network.
• Validate cross-reactivity in mouse or rat samples by comparing to human cell lysates, as the antibody is reported to react with all three species.
• Use appropriate controls (e.g., alpha-tubulin co-staining) to confirm microtubule localization in imaging experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. For microtubule and cytoskeleton research, we can supply this βII-Tubulin/β2-Tubulin rabbit monoclonal antibody alongside rare-target sourcing and technical support, bridging your project from concept to clinic with confidence.