Anti-α-Actinin-4 Polyclonal Antibody for WB, ELISA - O43707

Core Product Specifications and Parameters

Biological Background: α-Actinin-4 Function and Localization

• Alpha-actinin-4 is an F-actin cross-linking and bundling protein that anchors actin to intracellular structures.
• It participates in vesicular trafficking via association with the CART complex, necessary for efficient transferrin receptor recycling.
  Related references: PMID:15772161
• It is involved in tight junction assembly in epithelial cells through interaction with MICALL2, linking it to the actin cytoskeleton.
• Alpha-actinin-4 can function as a transcriptional coactivator, stimulating PPARG and RARA-mediated transcription.
  Related references: PMID:22351778
• Through IGSF8 association, it regulates immune synapse formation and is required for T-cell activation.
  Related references: PMID:22689882
• The protein localizes to the nucleus, cytoplasm, cell junctions, stress fibers, and perinuclear region.
• Widely expressed across tissues, it plays a central role in cytoskeletal organization and intracellular transport.
• Keywords include actin-binding, acetylation, phosphoprotein, and disease variant, with ACTN4 mutations linked to focal segmental glomerulosclerosis (FSGS).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 570–650) of human α-Actinin-4, which may confer high specificity.
• For Western blot, a band at approximately 105 kDa is expected; verify in the relevant sample system (human, mouse, or rat).
• In ELISA, consider direct coating with the recombinant protein or use as capture/detection antibody in sandwich format.
• When using for co-immunoprecipitation or IF, empirical validation is recommended due to the polyclonal nature.
• Include appropriate positive and negative controls to confirm reactivity and specificity across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with a reliable one-stop sourcing hub for IVD raw materials. For researchers studying cytoskeletal dynamics, membrane trafficking, or kidney disease, our portfolio includes validated antibodies like anti-α-Actinin-4, as well as bulk ancillary reagents and custom raw-material sourcing. Whether you need optimized monoclonal/polyclonal antibody pairs or rare target reagents, CamelBio bridges your concept-to-clinic development with comprehensive supply solutions.