Anti-βIII-Tubulin Rabbit Monoclonal Antibody for WB, IF, IHC-P, ELISA - Q13509

Core Product Specifications and Parameters

Biological Background: βIII-Tubulin Function and Localization

• As a beta-tubulin subunit, βIII-tubulin heterodimerizes with alpha-tubulin to form microtubules, with GTP-tubulin addition at growing ends and a stabilizing cap (PubMed:34996871, PubMed:38305685, PubMed:38609661). Related references: PMID:34996871, PMID:38305685, PMID:38609661
• TUBB3 is critical for proper axon guidance and maintenance, and mutations are associated with disorders like congenital fibrosis of the extraocular muscles (CFEOM3) (PubMed:20074521). Related references: PMID:20074521
• Binding of NTN1/Netrin-1 to its receptor UNC5C may dissociate UNC5C from polymerized TUBB3 microtubules, increasing dynamics and mediating axon repulsion; also involved in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977). Related references: PMID:28483977
• Subcellularly localized to the cytoplasm and cytoskeleton, and enriched in cell projections such as growth cones, lamellipodia, and filopodia.
• Expression is primarily restricted to the central and peripheral nervous system, with greatly increased levels in most cancerous tissues.
• Post-translational modifications include phosphorylation and isopeptide bond formation, and the protein is subject to alternative splicing.
• Genetic variants in TUBB3 are linked to intellectual disability, highlighting its role in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen, a synthetic peptide from residues 351-450 of human βIII-Tubulin, was designed to generate antibodies that recognize the native protein; consider using this region for peptide-blocking experiments to confirm specificity.
• For Western blotting, expect a band around 50 kDa; optimize lysate preparation from neuronal tissues or cell lines and include loading controls.
• In immunofluorescence applications, this antibody should stain filamentous cytoskeletal structures, particularly at growth cones and lamellipodia; permeabilization is required.
• For immunohistochemistry on paraffin-embedded sections, antigen retrieval may be necessary; positive control tissues include brain and neuronal tumors.
• The antibody’s reported cross-reactivity with mouse and rat makes it suitable for translational studies in rodent models.
• Validate the antibody in your specific sample system with a full dilution series, and consider isotype controls for multiplexed experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development from concept to clinic. This βIII-Tubulin rabbit monoclonal antibody exemplifies our portfolio for neuroscience and oncology applications, and we can support your projects with custom reagent sourcing, technical consultation, and streamlined supply of rare-target biomaterials. Let CamelBio bridge your procurement needs so you can focus on assay development and clinical translation.