SARS-CoV-2 NSP8 Rabbit Polyclonal Antibody for WB, ELISA - P0DTC2

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• Spike glycoprotein (S protein, also known as E2, Peplomer protein) is a major surface antigen of SARS-CoV-2, essential for viral entry into host cells. It exists as a homotrimer and is cleaved into S1 (receptor-binding) and S2 (membrane fusion) subunits. Related references: PMID:32142651 PMID:32155444 PMID:33607086

• S1 subunit attaches to host receptors, primarily angiotensin-converting enzyme 2 (ACE2), initiating infection. Binding triggers conformational changes leading to membrane fusion either directly at the cell surface or after endocytosis. Related references: PMID:34561887 PMID:32075877 PMID:32221306 PMID:34903715

• Alternative receptors, such as neuropilin-1 (NRP1) and NRP2, integrins, or ASGR1, may mediate entry in an ACE2-independent manner, potentially explaining viral tropism in olfactory epithelia with low ACE2 expression. Related references: PMID:33082294 PMID:33082293 PMID:35150743 PMID:34837059

• Cleavage of the S protein at the S2/S2' site by host proteases, including TMPRSS2 at the cell surface or cathepsin L in endosomes, is essential for priming the fusion machinery and releasing the viral genome into the cytoplasm. Related references: PMID:32142651 PMID:32703818 PMID:34159616

• As a class I viral fusion protein, S2 undergoes an irreversible conformational change from a pre-fusion state to a post-fusion hairpin structure, mediated by heptad repeat regions that form a trimer-of-hairpins, driving membrane fusion. Related references: PMID:34561887

• Structural studies reveal that the stalk domain of S contains flexible hinges, allowing orientational freedom of the head, which may facilitate receptor engagement and immune evasion. Related references: PMID:32817270

• The Spike protein is located on the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment membrane, and host cell membrane. It undergoes extensive post-translational modifications including glycosylation and palmitoylation, and is a key virulence factor.

• Key functional keywords include viral attachment to host cell, fusion of virus membrane with host membrane, viral envelope protein, host cell membrane, inhibition of host innate immune response, superantigen, and viral immunoevasion.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 1-198 of SARS-CoV-2 NSP8 (YP_009742615.1). For Western blot (WB), consider using appropriate positive controls such as SARS-CoV-2 infected cell lysates or recombinant NSP8. Optimization of antibody dilution and detection conditions is recommended.
• For ELISA, ensure proper coating with the immunogen or relevant NSP8-derived peptides. Validate the assay with positive and negative serum controls. The polyclonal nature may yield sensitive detection but could require careful titering to minimize background.
• As cross-reactivity is confirmed for SARS-CoV-2, the antibody may detect NSP8 from SARS-CoV-2 variants, but specific variant testing is advised. Consider epitope mapping if precise reactivity is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies for viral targets like SARS-CoV-2, and bulk ancillary reagents. With expertise spanning from concept to clinic, CamelBio supports diagnostic manufacturers in acquiring rare-target raw materials, such as antibodies against coronavirus non-structural proteins, ensuring supply chain reliability for assay development. For customized sourcing needs or technical consultation, our team is ready to assist.