Anti-SARS-CoV-2 Spike RBD (Omicron) Polyclonal Antibody for WB, ELISA - P0DTC2

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• The Spike glycoprotein (S) attaches to host cell receptor ACE2 via its receptor-binding domain (RBD) to initiate viral infection. Related references: PMID:32142651, PMID:32155444, PMID:33607086
• S can also use NRP1/NRP2 and integrins as alternative entry receptors, contributing to tissue tropism and olfactory epithelial cell infection. Related references: PMID:33082293, PMID:35150743
• Proteolytic cleavage at the S2/S2' site by host proteases TMPRSS2 or cathepsin L triggers irreversible conformational changes that drive fusion of viral and host membranes. Related references: PMID:32142651, PMID:32703818, PMID:34561887
• The Spike protein localizes to the virion membrane and is also trafficked to the host endoplasmic reticulum–Golgi intermediate compartment (ERGIC) and host cell membrane.
• S is synthesized as a precursor and processed into S1 and S2 subunits; S1 contains the RBD, while S2 anchors the fusion machinery and contains heptad repeat regions that form a trimer-of-hairpins during fusion. Related references: PMID:34561887
• Keywords associated with S include viral envelope protein, host-virus interaction, viral immunoevasion, and superantigen activity, reflecting its multifaceted roles in infection and pathogenicity.
• The full-length Spike protein has a calculated molecular weight of about 141 kDa; the immunogen corresponds to the Omicron variant RBD (amino acids 319–541).

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 319–541 of the Spike RBD from the Omicron variant (P0DTC2). For Western blot (WB), consider using reducing conditions and validate with recombinant RBD protein or lysates from SARS-CoV-2-infected cells.
• In ELISA, this polyclonal antibody can be used as a capture or detection reagent. Pairing with a matched monoclonal antibody can improve specificity. Titration and optimization are recommended for each assay format.
• As the antibody is reactive with SARS-CoV-2, cross-reactivity with other coronaviruses or SARS-CoV-2 variants should be verified experimentally if required.
• For applications not listed (e.g., immunofluorescence, immunohistochemistry), the suitability must be determined by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of raw materials for the IVD industry, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-Spike RBD polyclonal antibody complements our portfolio of SARS-CoV-2-related reagents, and we can source validated antibody pairs, recombinant proteins, and ancillary reagents for your COVID-19 assay development. Reach out for flexible, scalable procurement solutions tailored to your project needs.