Anti-SARS-CoV Spike RBD Polyclonal Antibody for WB, ELISA - P59594

Core Product Specifications and Parameters

Biological Background: SARS-CoV Spike RBD Function and Localization

• The SARS-CoV Spike glycoprotein (also known as E2 or Peplomer protein) is encoded by the S gene and is essential for viral entry into host cells.
• It mediates viral attachment by binding to host receptors angiotensin-converting enzyme 2 (ACE2) and CLEC4M/DC-SIGNR, triggering internalization of the virion into endosomes.
• Following receptor binding and proteolytic activation by host cathepsins, the Spike protein acts as a class I viral fusion protein, driving fusion between the viral and host cell membranes.
• The receptor-binding domain (RBD) located within the S1 subunit is directly responsible for ACE2 interaction and is a major target for neutralizing antibodies.
• The protein also exhibits immunomodulatory functions, such as down-regulating host tetherin (BST2) via lysosomal degradation to counteract antiviral innate immune responses.
• Subcellularly, the Spike glycoprotein localizes to the virion membrane, host endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane, and host cell membrane.
• It is extensively post-translationally modified, featuring glycosylation, disulfide bonds, and palmitoylation, which are critical for its structure and function.
• Additional keywords: viral immunoevasion, inhibition of host innate immune response, and virulence factor.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide spanning amino acids 350-450 of the Spike RBD (based on accession NP_828851.1); consider this epitope region when designing detection assays.
• This polyclonal antibody is suitable for Western blot (WB) and ELISA applications; users should optimize dilution factors and validate performance in their specific sample matrices.
• Due to the noted cross-reactivity with SARS-CoV, the antibody may also recognize Spike proteins from closely related strains, but reactivity with other coronaviruses should be assessed experimentally.
• For WB detection, the predicted molecular weight of the full-length Spike glycoprotein is approximately 139 kDa, but cleavage products or degradation bands may be observed depending on sample preparation.
• In ELISA, pairing this antibody with a suitable capture antibody or antigen coating strategy targeting the RBD can enhance assay sensitivity; consider preliminary checkerboard titrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents for infectious disease diagnostics. Whether you are developing serological assays for SARS-CoV-2 or research-grade tests for SARS-like coronaviruses, we provide reliable access to validated polyclonal antibodies and can support custom bulk requests. From concept to clinic, count on CamelBio to streamline your supply chain with high-quality, traceable materials.