Anti-SARS-CoV-2 N Protein Rabbit Polyclonal Antibody for WB, ELISA - P59595

Core Product Specifications and Parameters

Biological Background: Nucleoprotein Function and Localization

• The nucleoprotein (N protein) packages the positive-strand viral RNA genome into a helical ribonucleocapsid, essential for viral genome protection and organization.
• N protein interacts with the viral membrane protein M to mediate virion assembly.
• It enhances the efficiency of subgenomic viral RNA transcription and viral replication. Related references: PMID:17210170
• N protein may modulate host transforming growth factor-beta signaling by binding to host SMAD3. Related references: PMID:18055455
• Subcellular localization: found in the mature virion and in host cell compartments including the cytoplasm, perinuclear region, Golgi apparatus, endoplasmic reticulum-Golgi intermediate compartment, and nucleus.
• Post-translational modifications include ADP-ribosylation and phosphorylation (phosphoprotein), as indicated by UniProt keywords.
• The protein has a molecular weight of approximately 46 kDa and is classified as an RNA-binding ribonucleoprotein.

Experimental Guidance and Technical Tips

• For western blotting, the expected molecular weight of SARS-CoV-2 N protein is approximately 46 kDa; consider using appropriate positive controls and molecular weight markers.
• This polyclonal antibody, raised against the N-terminal region (aa 1-100), may recognize both full-length N protein and potential N-terminal fragments; validation in the relevant sample system is recommended.
• For ELISA, optimal coating concentrations and detection conditions should be empirically determined using recombinant or native N protein.
• The antibody is reported to detect SARS-CoV-2 N protein; cross-reactivity with N proteins from other coronaviruses should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This SARS-CoV-2 N protein polyclonal antibody is part of our comprehensive infectious disease portfolio, supporting virology and vaccine development research. Our supply chain expertise ensures consistent access to rare and specialized raw materials to accelerate your assay development from concept to clinic.