Anti-REST/NRSF Rabbit Polyclonal Antibody for WB, ELISA - Q13127

Core Product Specifications and Parameters

Biological Background: REST/NRSF Function and Localization

• Functions as a transcriptional repressor that binds to the neuron-restrictive silencer element (NRSE) and recruits corepressors SIN3A and RCOR1, leading to histone deacetylase recruitment and silencing of neuronal genes in non-neuronal cells. Related references: PMID:11741002, PMID:11779185, PMID:12399542, PMID:10449787, PMID:10734093

• Mediates chromatin modification through the BHC complex, which deacetylates and demethylates histones at RE1/NRSE sites to enforce gene silencing.

• Represses transcription via CDYL-dependent recruitment of histone methyltransferase EHMT2, a mechanism that may suppress cellular transformation. Related references: PMID:19061646

• Repressor activity is antagonized by heterodimerization with the alternatively spliced isoform 3, which prevents NRSE binding and derepresses target genes. Related references: PMID:11779185

• Maintains the quiescent state of adult neural stem cells by repressing neuronal genes; dissociation from NRSE upon differentiation cues allows neuron specification. Related references: PMID:21258371

• Acts as a key repressor of gene expression under hypoxic conditions and contributes to stress resistance and cell death regulation in the aging brain. Related references: PMID:27531581, PMID:24670762

• Tissue expression: detected in neurons of the prefrontal cortex, hippocampus, and cerebellum; nuclear levels are reduced in Alzheimer disease and Parkinson disease, with pathological aggregation in Lewy bodies in dopaminergic neurons. Related references: PMID:24670762, PMID:30684677

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–130 of human REST, an N-terminal region N-terminal to the zinc finger domains. Users should verify that their experimental system preserves this epitope.
• For Western blot, start with a dilution of 1:500–1:2000 and validate using lysates from non-neuronal REST-expressing cell lines (e.g., HeLa) alongside appropriate controls.
• For ELISA, recombinant REST protein or peptide spanning the immunogen region can serve as a positive control to assess antibody binding.
• Although cross-reactivity with mouse and rat is reported, sequence alignment across species in the immunogen region is recommended to confirm expected reactivity.
• Due to the large molecular weight (~122 kDa), ensure gel electrophoresis conditions are optimized for high-molecular-weight proteins, and consider extended transfer times in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies, validated pairs, and bulk ancillary reagents. For transcriptional regulators like REST/NRSF—central to neuroscience and oncology research—we offer comprehensive supply solutions to accelerate your assay development from concept to clinic. Our portfolio includes polyclonal antibodies, matched detection pairs, and custom sourcing for rare targets, ensuring you have reliable raw materials at every stage.