Anti-SARS-CoV-2 NSP2 Rabbit Polyclonal Antibody for WB and ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 NSP2 Function and Localization

• NSP2 is a nonstructural protein cleaved from the replicase polyprotein 1ab (ORF1ab) of SARS-CoV-2, a large multifunctional precursor involved in viral transcription and replication.
• NSP2 enhances host mRNA repression by the 4EHP-GYF2 complex, thereby inhibiting antiviral responses and facilitating viral replication. This activity occurs in cooperation with NSP1, which induces ribosome stalling on host transcripts.
• NSP2 primarily localizes to the host cytoplasm and is associated with viral replication complexes that form on endoplasmic reticulum-derived double-membrane vesicles.
• Unlike many other nonstructural proteins, NSP2 lacks known enzymatic domains; its role in host immune antagonism appears to rely on protein–protein interactions, particularly through modulation of the 4EHP-GYF2 pathway.
• The polyprotein also generates other critical enzymes: the papain-like protease (nsp3) that cleaves NSP2 from the precursor, the main protease (nsp5), and the RNA-dependent RNA polymerase (nsp12).

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning residues 150–250 of NSP2. In Western blot, the mature NSP2 protein is predicted to migrate at ~71 kDa; however, the full-length replicase polyprotein (794 kDa) may also be visible if processing is incomplete. Validate the detected band(s) using appropriate controls (e.g., SARS-CoV-2-infected cell lysates vs. mock).
• For ELISA, consider coating with recombinant NSP2 protein or viral lysate. Perform titration experiments to determine optimal working dilutions.
• Cross-reactivity is confirmed against SARS-CoV-2; testing against other coronaviruses or related NSP2 homologs is recommended for off-target assessment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to supply high-quality IVD raw materials, including antibodies for emerging infectious diseases like SARS-CoV-2. This anti-NSP2 rabbit polyclonal antibody is suitable for viral replication and immune evasion studies. Contact us for bulk orders, custom conjugations, and technical support to streamline your assay development.