Anti-SARS-CoV-2 NSP9 Rabbit Polyclonal Antibody for WB, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: NSP9 Function and Localization

• NSP9 is a non-structural protein encoded by the SARS-CoV-2 replicase polyprotein 1ab (pp1ab), liberated from the polyprotein through proteolytic processing by viral proteases.
• It forms a covalent RNA-protein intermediate with the NiRAN domain of NSP12, serving as a primer for the initiation of RNA synthesis by the viral RNA-dependent RNA polymerase. Related references: PMID:37794589
• NSP9 interacts with the ribosome signal recognition particle RNA (SRP) and, together with NSP8, suppresses host protein integration into the cell membrane, thereby disrupting immune defenses. Related references: PMID:33080218
• NSP9 plays a critical role in viral RNA replication and transcription, potentially participating in the formation of protein complexes essential for viral propagation.
• The protein localizes predominantly to the host cytoplasm and perinuclear regions, likely associated with viral replication organelles.
• As a highly conserved coronavirus protein, NSP9 represents an attractive target for antiviral research and diagnostic development.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the N-terminal region (amino acids 1-113) of SARS-CoV-2 NSP9; this antibody may detect both the processed NSP9 and the full-length polyprotein precursor.
• For Western blot, appropriate samples include SARS-CoV-2-infected cell lysates or recombinant NSP9 protein; a band around ~12 kDa (processed NSP9) or ~794 kDa (polyprotein) may be observed depending on sample preparation.
• For ELISA, the antibody can be used as a capture or detection reagent; validate with recombinant NSP9 protein or viral lysates in your assay system.
• As this is a polyclonal antibody, consider batch-to-batch variability and validate performance in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibodies for infectious disease targets like SARS-CoV-2 NSP9. Our portfolio encompasses antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to support viral research and diagnostic development. From bulk supply to rare target sourcing, CamelBio offers reliable procurement solutions tailored to your project needs.