Anti-SARS-CoV-2 NSP3 Polyclonal Antibody for WB, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: SARS-CoV-2 NSP3 Function and Localization

• SARS-CoV-2 NSP3 is a component of the replicase polyprotein 1ab (also known as ORF1ab polyprotein) that is cleaved by its own papain-like protease activity to release the NSP3 protein.
• NSP3 possesses papain-like protease (PL-PRO) activity responsible for cleavages at the N-terminus of the polyprotein, and collaborates with NSP4 to assemble double-membrane vesicles (DMVs) for viral replication (PubMed:35551511). Related references: PMID:35551511
• It antagonizes innate immune induction of type I interferon by blocking phosphorylation, dimerization, and nuclear translocation of host IRF3 (PubMed:32733001), and prevents NF-kappa-B signaling (By similarity). Related references: PMID:32733001
• NSP3 acts as a deubiquitinating and deISGylating enzyme, processing both Lys-48- and Lys-63-linked polyubiquitin chains, and preferentially removing ISG15 from IFIH1/MDA5 (PubMed:32726803, PubMed:33727702). Related references: PMID:32726803, PMID:33727702
• The protein exhibits ADP-ribose hydrolase activity, removing mono-ADP-ribose from host proteins, likely interfering with host PARP-mediated immune defenses (Probable) (PubMed:37242344). Related references: PMID:37242344
• Subcellular localization includes host cytoplasm, endoplasmic reticulum membrane, and double-membrane vesicles, consistent with its role in replication organelle formation.
• Keywords include viral immunoevasion, inhibition of host innate immune response, and deubiquitinase activity, highlighting its multifunctional role in host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-200 of NSP3 (YP_009725299.1), a region within the N-terminal domain; this may capture epitopes critical to its protease or ADP-ribose binding functions.
• For Western blot (WB), consider using whole-cell lysates from SARS-CoV-2 infected cells, but validate the expected band (approximately 794 kDa for the full polyprotein or cleaved NSP3 fragment) in your specific system.
• ELISA applications can be developed using this polyclonal antibody as capture or detection reagent; pairing with a monoclonal antibody may improve specificity.
• Cross-reactivity has been confirmed with SARS-CoV-2; testing with other coronaviruses is recommended if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD manufacturers, research labs, and diagnostic developers. This SARS-CoV-2 NSP3 polyclonal antibody supports viral replication and immune evasion studies, and is part of our broad portfolio of validated raw materials. We also provide customized sourcing, technical consulting, and bulk supply services for rare targets and ancillary reagents.