SARS-CoV-2 NSP10 Rabbit Polyclonal Antibody for WB, IP, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: NSP10 Function and Localization

• NSP10 is a non-structural protein encoded by the SARS-CoV-2 replicase gene (rep) as part of the ORF1ab polyprotein (Replicase polyprotein 1ab, P0DTD1). It is released by proteolytic cleavage of the polyprotein.
• NSP10 plays a pivotal role in viral transcription by stimulating both the nsp14 3'-5' exoribonuclease (proofreading) and nsp16 2'-O-methyltransferase activities, thereby ensuring proper viral mRNA cap methylation.
  Related references: PMID:35944563
• NSP10 localizes predominantly to the host cytoplasm and perinuclear region, where it interacts with other non-structural proteins to form the replication-transcription complex (RTC).
• As a critical cofactor, NSP10 is essential for viral RNA capping, which helps the virus evade host innate immune sensors such as IFIT1 and IFIT3.
• The protein is highly conserved among coronaviruses, making it a potential target for antiviral therapeutics and a relevant antigen for antibody development.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant protein covering amino acids 1–139 of SARS-CoV-2 NSP10 (YP_009725306.1). This full-length construct is expected to generate antibodies recognizing multiple epitopes, offering robust detection in assays.
• Polyclonal antibodies may exhibit slight lot-to-lot variability; it is advisable to titrate and validate the antibody in the specific experimental system (e.g., infected cell lysates for WB, IP, or ELISA).
• For Western blot, consider using appropriate positive controls such as SARS-CoV-2 infected cell lysates or overexpressed NSP10. Note that NSP10 migrates at ~14 kDa, while the reported 794 kDa reflects the full-length replicase polyprotein.
• In immunoprecipitation, the antibody may co-immunoprecipitate interacting partners like nsp14 and nsp16; validate co-IP conditions carefully.
• For ELISA, consider using recombinant NSP10 protein as a standard to assess antibody sensitivity and specificity before testing with clinical samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutes streamlined access to validated antibodies, optimized recombinant proteins, and ancillary reagents. This SARS-CoV-2 NSP10 polyclonal antibody serves researchers studying coronavirus replication and mRNA capping mechanisms. CamelBio can also assist in sourcing rare targets and providing technical consultation to accelerate your assay development from concept to clinic.