SARS-CoV-2 NSP1 Rabbit pAb - P0DTC2

Core Product Specifications and Parameters

Biological Background: Spike glycoprotein Function and Localization

• The spike (S) glycoprotein mediates virus entry by binding to the host receptor angiotensin-converting enzyme 2 (ACE2) and triggering membrane fusion. Related references: PMID:32142651, PMID:32155444, PMID:33607086
• Cleavage of S protein at the S2/S2' site by host proteases TMPRSS2 or cathepsin L is essential for virus entry, either at the plasma membrane or within endosomes. Related references: PMID:32142651, PMID:32703818, PMID:34159616
• Alternative receptors such as neuropilin-1 (NRP1), NRP2, integrins, and ASGR1 may facilitate virus entry in specific tissues. Related references: PMID:33082294, PMID:33082293, PMID:35150743, PMID:34837059
• S protein undergoes an irreversible conformational change from a pre-fusion to a post-fusion state, mediated by heptad repeat regions, culminating in membrane fusion. Related references: PMID:34561887
• The S protein localizes to the virion membrane, host ER-Golgi intermediate compartment, and host cell membrane.
• It is extensively glycosylated and contains multiple disulfide bonds, coiled-coil motifs, and palmitoylation sites.
• Additional functions include inhibition of host innate immune response and tetherin, and potential superantigen activity.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region of NSP1, suggesting suitability for detecting full-length and potentially truncated forms.
• For Western blot, consider using appropriate positive controls such as SARS-CoV-2-infected cell lysates and optimize blocking conditions to reduce background.
• In immunoprecipitation experiments, pre-clearing lysates and including appropriate isotype controls may improve specificity.
• For ELISA, titrate the antibody to determine the optimal coating concentration and validate with known antigen standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For SARS-CoV-2 research and assay development, we offer one-stop sourcing for NSP1 antibodies and related viral targets. Our supply capabilities also encompass rare target raw-material procurement to support your diagnostic pipeline from concept to clinic.