Anti-SARS-CoV-2 NSP4 Rabbit Polyclonal Antibody for WB, IP, ELISA - P0DTD1

Core Product Specifications and Parameters

Biological Background: NSP4 Function and Localization

• SARS-CoV-2 non-structural protein 4 (NSP4) is a component of the replicase polyprotein 1ab (pp1ab) encoded by the ORF1ab gene.
• NSP4 is essential for the formation of double-membrane vesicles (DMVs), the viral replication organelles, in conjunction with NSP3.
  Related references: PMID:35551511
• DMVs provide a protective microenvironment for viral RNA synthesis, shielding it from host innate immune sensors.
• NSP4 localizes to the host endoplasmic reticulum (ER) membrane, where it induces membrane curvature and vesiculation.
• It is a multi-spanning transmembrane protein, integrating into the ER membrane via several hydrophobic regions.
• NSP4 function is conserved among coronaviruses, highlighting its importance in the viral replication cycle.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (aa 400–500) of NSP4, predicted to be on the cytoplasmic side of the ER membrane; this design may facilitate detection of both denatured and native protein in applications such as WB and IP.
• For Western blot, be aware that the mature NSP4 protein migrates at approximately 56 kDa, while the full-length pp1ab polyprotein is ~794 kDa; optimize gel percentage and transfer conditions accordingly.
• In ELISA, consider coating with recombinant NSP4 protein or pairing this antibody with a suitable detection antibody for sandwich assays; validation in SARS-CoV-2 infected cell lysates is advised.
• Since the antibody cross-reacts with SARS-CoV-2, it is suitable for studying this specific coronavirus; test for cross-reactivity to other coronaviruses if broader applications are required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. This anti-SARS-CoV-2 NSP4 pAb is a valuable tool for studying viral replication organelle formation and developing COVID-19 diagnostic or research assays. CamelBio offers comprehensive supply capabilities including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing for rare targets, ensuring seamless procurement for your IVD projects.